Detailed information of TA system TA114282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2887717..2888444
• Replicon: chromosome
• Accession: NZ_CP033762
• Organism: Escherichia coli strain FDAARGOS_536

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2R9W4D2
• Locus tag: EGX93_RS15090
• Protein ID: WP_000550184.1
• Coordinates: 2888130..2888444 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGX93_RS15085
• Protein ID: WP_000560266.1
• Coordinates: 2887717..2888133 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX93_RS15075	2883077..2885428	+	2352	WP_042020082.1	alpha-glucosidase	-
EGX93_RS15080	2885654..2887672	+	2019	WP_042020084.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
EGX93_RS15085	2887717..2888133	-	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
EGX93_RS15090	2888130..2888444	-	315	WP_000550184.1	type II toxin-antitoxin system toxin HigB	Toxin
EGX93_RS15095	2888730..2889866	-	1137	WP_000018671.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
EGX93_RS15100	2889951..2890454	+	504	WP_001295542.1	M48 family metallopeptidase	-
EGX93_RS15105	2890531..2891223	+	693	WP_024231607.1	vancomycin high temperature exclusion protein	-
EGX93_RS15110	2891302..2892288	+	987	WP_024231606.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12073.08 Da
• Isoelectric Point: 10.0409

>T114282 WP_000550184.1 NZ_CP033762:c2888444-2888130 [Escherichia coli]
MHLITQKALKDAAEKYPQHKAELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T114282 NZ_CP033762:c2888444-2888130 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAGCGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGAGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT114282 WP_000560266.1 NZ_CP033762:c2888133-2887717 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT114282 NZ_CP033762:c2888133-2887717 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGTTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCGCAAGCCATGCCTGGCGGT
ATAGCTGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A2R9W4D2