Detailed information of TA system TA114278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2354647..2355374
• Replicon: chromosome
• Accession: NZ_CP033762
• Organism: Escherichia coli strain FDAARGOS_536

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: EGX93_RS12545
• Protein ID: WP_000547563.1
• Coordinates: 2355063..2355374 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGX93_RS12540
• Protein ID: WP_000126304.1
• Coordinates: 2354647..2355066 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX93_RS12520	2350810..2353062	-	2253	WP_021522198.1	carbamoyltransferase HypF	-
EGX93_RS12525	2353215..2353742	-	528	WP_001078777.1	electron transport protein HydN	-
EGX93_RS12535	2354127..2354555	+	429	WP_000536064.1	hypothetical protein	-
EGX93_RS12540	2354647..2355066	-	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
EGX93_RS12545	2355063..2355374	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
EGX93_RS12550	2355537..2356292	-	756	WP_001553332.1	hypothetical protein	-
EGX93_RS12555	2356335..2356805	-	471	WP_000132956.1	hydrogenase maturation peptidase HycI	-
EGX93_RS12560	2356798..2357208	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EGX93_RS12565	2357205..2357972	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
EGX93_RS12570	2357972..2358514	-	543	WP_000493799.1	formate hydrogenlyase subunit HycF	-
EGX93_RS12575	2358524..2360233	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T114278 WP_000547563.1 NZ_CP033762:c2355374-2355063 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T114278 NZ_CP033762:c2355374-2355063 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT114278 WP_000126304.1 NZ_CP033762:c2355066-2354647 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT114278 NZ_CP033762:c2355066-2354647 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22