Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1604979..1605481 Replicon chromosome
Accession NZ_CP033762
Organism Escherichia coli strain FDAARGOS_536

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag EGX93_RS08970 Protein ID WP_000767826.1
Coordinates 1604979..1605233 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EGX93_RS08975 Protein ID WP_001259255.1
Coordinates 1605230..1605481 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX93_RS08940 1599994..1600221 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EGX93_RS08945 1600235..1601293 - 1059 WP_000492324.1 transport protein YeeE -
EGX93_RS08950 1601472..1602830 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EGX93_RS08955 1602820..1602882 - 63 WP_010723108.1 membrane protein YoeI -
EGX93_RS08960 1603097..1604026 - 930 WP_000803363.1 LysR family transcriptional regulator -
EGX93_RS08965 1604072..1604896 - 825 WP_000754737.1 SDR family oxidoreductase -
EGX93_RS08970 1604979..1605233 - 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EGX93_RS08975 1605230..1605481 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EGX93_RS08980 1605764..1605814 + 51 WP_001364200.1 his operon leader peptide -
EGX93_RS08985 1605960..1606859 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EGX93_RS08990 1606865..1608169 + 1305 WP_042020206.1 histidinol dehydrogenase -
EGX93_RS08995 1608166..1609236 + 1071 WP_042020204.1 histidinol-phosphate transaminase -
EGX93_RS09000 1609236..1610303 + 1068 WP_042020202.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T114277 WP_000767826.1 NZ_CP033762:c1605233-1604979 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T114277 NZ_CP033762:c1605233-1604979 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT114277 WP_001259255.1 NZ_CP033762:c1605481-1605230 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT114277 NZ_CP033762:c1605481-1605230 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References