Detailed information of TA system TA114241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 32781..33505
• Replicon: plasmid unnamed1
• Accession: NZ_CP033755
• Organism: Klebsiella pneumoniae strain FDAARGOS_566

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: EGY03_RS00230
• Protein ID: WP_023292165.1
• Coordinates: 32781..33092 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGY03_RS00235
• Protein ID: WP_049010942.1
• Coordinates: 33089..33505 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY03_RS00210	28789..29691	-	903	WP_060598839.1	LysR family transcriptional regulator	-
EGY03_RS00215	29974..31113	+	1140	WP_072122725.1	PLP-dependent aspartate aminotransferase family protein	-
EGY03_RS00220	31134..32063	+	930	WP_158236771.1	DMT family transporter	-
EGY03_RS00225	32178..32479	+	302	Protein_39	DDE-type integrase/transposase/recombinase	-
EGY03_RS00230	32781..33092	+	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
EGY03_RS00235	33089..33505	+	417	WP_049010942.1	helix-turn-helix domain-containing protein	Antitoxin
EGY03_RS00240	34322..34702	+	381	WP_060598880.1	hypothetical protein	-
EGY03_RS00245	34769..35116	+	348	WP_060598879.1	hypothetical protein	-
EGY03_RS00250	35208..35438	+	231	WP_023340951.1	hypothetical protein	-
EGY03_RS00255	35485..36318	+	834	WP_060598878.1	type I restriction-modification system subunit M	-
EGY03_RS00260	36943..37473	+	531	WP_015632491.1	antirestriction protein	-
EGY03_RS00265	37511..37990	-	480	WP_060598877.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..103852	103852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T114241 WP_023292165.1 NZ_CP033755:32781-33092 [Klebsiella pneumoniae]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T114241 NZ_CP033755:32781-33092 [Klebsiella pneumoniae]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCAAGACACTATCCGAATGATGCAGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAGAAATGGTGGGTTATCGATATCGGCGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15438.52 Da
• Isoelectric Point: 4.4711

>AT114241 WP_049010942.1 NZ_CP033755:33089-33505 [Klebsiella pneumoniae]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT114241 NZ_CP033755:33089-33505 [Klebsiella pneumoniae]
ATGATTTTTAGCGATGCCATTAAAGCTGCGAATGACCTGGCCAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTCGAAAATGAGATAGGTAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3