Detailed information of TA system TA114228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1488498..1489197
• Replicon: chromosome
• Accession: NZ_CP033748
• Organism: Burkholderia multivorans strain FDAARGOS_547

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: J4RFZ0
• Locus tag: EGY20_RS06845
• Protein ID: WP_006413131.1
• Coordinates: 1488498..1488794 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: EGY20_RS06850
• Protein ID: WP_048804721.1
• Coordinates: 1488796..1489197 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY20_RS06830	1485219..1486763	+	1545	WP_172964954.1	sensor domain-containing diguanylate cyclase	-
EGY20_RS06835	1486969..1487241	+	273	WP_012216969.1	hypothetical protein	-
EGY20_RS06840	1487344..1488378	+	1035	WP_012216968.1	succinylglutamate desuccinylase/aspartoacylase family protein	-
EGY20_RS06845	1488498..1488794	+	297	WP_006413131.1	type II toxin-antitoxin system MqsR family toxin	Toxin
EGY20_RS06850	1488796..1489197	+	402	WP_048804721.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
EGY20_RS06855	1489486..1490046	+	561	WP_060151330.1	GNAT family N-acetyltransferase	-
EGY20_RS06865	1490112..1491053	+	942	WP_039217954.1	ABC transporter substrate-binding protein	-
EGY20_RS06870	1491085..1492146	+	1062	WP_123789377.1	LLM class flavin-dependent oxidoreductase	-
EGY20_RS06875	1492143..1493495	+	1353	WP_060144883.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11199.10 Da
• Isoelectric Point: 9.1122

>T114228 WP_006413131.1 NZ_CP033748:1488498-1488794 [Burkholderia multivorans]
MEKRTPHCKLPRVKALIEAGKVRLTASAVIGARQMGFTEAETIDVVRALTPFEFHKSMTTYADHTIWQDVYRPCTMRGDV
YLKLTVVDDVLIVSFKER

Nucleotide

• Length: 297 bp

>T114228 NZ_CP033748:1488498-1488794 [Burkholderia multivorans]
ATGGAAAAGAGAACGCCGCATTGCAAGCTGCCGCGCGTCAAGGCGCTGATCGAAGCCGGCAAGGTCCGCCTGACCGCAAG
CGCCGTCATCGGCGCCCGCCAGATGGGCTTCACCGAAGCGGAAACGATCGACGTGGTGAGGGCGCTGACACCGTTCGAAT
TCCACAAGAGCATGACGACCTACGCCGACCACACGATCTGGCAGGACGTCTACCGGCCTTGCACGATGCGCGGCGACGTG
TATCTGAAACTGACCGTCGTCGACGATGTGCTGATCGTGTCTTTCAAGGAGCGATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14521.68 Da
• Isoelectric Point: 7.0834

>AT114228 WP_048804721.1 NZ_CP033748:1488796-1489197 [Burkholderia multivorans]
MKCPACGAATLIRDTRDIPYTYKGESTVIPHVTGEFCPACGESVLDVDEATRFGEAVSAFNRQVNATLVDPKFIATVRKK
LKLDQREAAEIFGGGVNAFSRYETGKTNPPLALVKLLKLLDRHPDLLDEVRAA

Nucleotide

• Length: 402 bp

>AT114228 NZ_CP033748:1488796-1489197 [Burkholderia multivorans]
ATGAAATGTCCTGCATGCGGCGCCGCCACGCTGATCCGCGATACGCGCGACATCCCGTACACCTACAAGGGCGAATCGAC
CGTGATCCCGCACGTCACCGGCGAGTTTTGCCCCGCGTGCGGCGAGTCGGTGCTCGACGTCGACGAGGCGACCCGTTTCG
GCGAAGCGGTGTCGGCGTTCAATCGGCAAGTGAATGCGACACTCGTCGATCCGAAGTTCATTGCGACGGTGCGCAAGAAG
CTGAAGCTCGATCAGCGCGAGGCGGCCGAGATTTTCGGCGGCGGCGTCAACGCGTTTTCCCGATACGAGACCGGCAAGAC
CAATCCGCCGCTGGCGCTCGTGAAGCTGCTGAAGCTTCTCGACCGTCATCCCGATCTGCTCGACGAAGTGCGCGCGGCAT
AA

Structures

Toxin

• AlphaFold DB: A0A132ERQ0