Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2473766..2474414 Replicon chromosome
Accession NZ_CP033742
Organism Citrobacter freundii strain FDAARGOS_550

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag EGY10_RS12180 Protein ID WP_016151211.1
Coordinates 2474052..2474414 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B5QFP8
Locus tag EGY10_RS12175 Protein ID WP_003844470.1
Coordinates 2473766..2474065 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY10_RS12155 2470087..2470504 - 418 Protein_2320 GNAT family N-acetyltransferase -
EGY10_RS12160 2470635..2470928 + 294 WP_008323809.1 hypothetical protein -
EGY10_RS12165 2471023..2472405 + 1383 WP_106902615.1 glycoside hydrolase family 1 protein -
EGY10_RS12170 2472454..2473647 - 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
EGY10_RS12175 2473766..2474065 - 300 WP_003844470.1 helix-turn-helix transcriptional regulator Antitoxin
EGY10_RS12180 2474052..2474414 - 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGY10_RS12185 2474515..2474967 - 453 WP_003023933.1 DUF1198 family protein -
EGY10_RS12190 2475093..2476484 - 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
EGY10_RS12195 2476627..2477955 - 1329 WP_003023927.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T114196 WP_016151211.1 NZ_CP033742:c2474414-2474052 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

>T114196 NZ_CP033742:c2474414-2474052 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11032.85 Da        Isoelectric Point: 8.5047

>AT114196 WP_003844470.1 NZ_CP033742:c2474065-2473766 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQSDNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp

>AT114196 NZ_CP033742:c2474065-2473766 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAACCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGTGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGTCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A8B5QFP8

References