Detailed information of TA system TA114055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14955..15595
• Replicon: plasmid unnamed2
• Accession: NZ_CP033698
• Organism: Yersinia pestis strain FDAARGOS_601

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EGX46_RS00580
• Protein ID: WP_002225538.1
• Coordinates: 14955..15296 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: EGX46_RS00585
• Protein ID: WP_002213446.1
• Coordinates: 15296..15595 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX46_RS00560	10657..10851	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-
EGX46_RS00565	11887..12669	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
EGX46_RS00570	12666..13688	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
EGX46_RS00580	14955..15296	+	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX46_RS00585	15296..15595	+	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
EGX46_RS00590	15782..16720	-	939	WP_002218234.1	omptin family plasminogen activator Pla	-
EGX46_RS00595	16963..17268	-	306	WP_002221218.1	hypothetical protein	-
EGX46_RS00600	17497..18570	+	1074	WP_002218509.1	pesticin	-
EGX46_RS00605	18605..19030	-	426	WP_002218512.1	pesticin immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla / pla	1..19216	19216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T114055 WP_002225538.1 NZ_CP033698:14955-15296 [Yersinia pestis]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T114055 NZ_CP033698:14955-15296 [Yersinia pestis]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT114055 WP_002213446.1 NZ_CP033698:15296-15595 [Yersinia pestis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT114055 NZ_CP033698:15296-15595 [Yersinia pestis]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016