Detailed information of TA system TA114054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5346..5986
• Replicon: plasmid unnamed2
• Accession: NZ_CP033698
• Organism: Yersinia pestis strain FDAARGOS_601

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EGX46_RS00525
• Protein ID: WP_002225538.1
• Coordinates: 5346..5687 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: EGX46_RS00530
• Protein ID: WP_002213446.1
• Coordinates: 5687..5986 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX46_RS00500	1048..1242	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-
EGX46_RS00510	2278..3060	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
EGX46_RS00515	3057..4079	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
EGX46_RS00525	5346..5687	+	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX46_RS00530	5687..5986	+	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
EGX46_RS00535	6173..7111	-	939	WP_002218234.1	omptin family plasminogen activator Pla	-
EGX46_RS00540	7354..7659	-	306	WP_002221218.1	hypothetical protein	-
EGX46_RS00545	7888..8961	+	1074	WP_002218509.1	pesticin	-
EGX46_RS00550	8996..9421	-	426	WP_002218512.1	pesticin immunity protein	-
EGX46_RS00560	10657..10851	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla / pla	1..19216	19216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T114054 WP_002225538.1 NZ_CP033698:5346-5687 [Yersinia pestis]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T114054 NZ_CP033698:5346-5687 [Yersinia pestis]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT114054 WP_002213446.1 NZ_CP033698:5687-5986 [Yersinia pestis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT114054 NZ_CP033698:5687-5986 [Yersinia pestis]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016