114018

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5617287..5617882 Replicon chromosome
Accession NZ_CP033686
Organism Pseudomonas aeruginosa strain H25883

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag EGV93_RS27005 Protein ID WP_003113526.1
Coordinates 5617604..5617882 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag EGV93_RS27000 Protein ID WP_003111575.1
Coordinates 5617287..5617592 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGV93_RS26970 5612299..5612571 - 273 WP_003115921.1 hypothetical protein -
EGV93_RS26980 5613346..5615232 + 1887 WP_071550095.1 putative DNA binding domain-containing protein -
EGV93_RS26990 5615739..5616071 + 333 WP_024947075.1 hypothetical protein -
EGV93_RS26995 5616331..5616945 + 615 WP_123794473.1 hypothetical protein -
EGV93_RS27000 5617287..5617592 - 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
EGV93_RS27005 5617604..5617882 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGV93_RS27015 5618211..5620439 + 2229 WP_031686603.1 TonB-dependent receptor -
EGV93_RS27020 5620510..5621157 - 648 WP_003095021.1 carbonate dehydratase -
EGV93_RS27025 5621219..5622457 - 1239 WP_031686604.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T114018 WP_003113526.1 NZ_CP033686:c5617882-5617604 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T114018 NZ_CP033686:c5617882-5617604 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCTGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT114018 WP_003111575.1 NZ_CP033686:c5617592-5617287 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT114018 NZ_CP033686:c5617592-5617287 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References