Detailed information of TA system TA114012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5669271..5669866
• Replicon: chromosome
• Accession: NZ_CP033685
• Organism: Pseudomonas aeruginosa strain H26023

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: EGV94_RS27110
• Protein ID: WP_003113526.1
• Coordinates: 5669588..5669866 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGV94_RS27105
• Protein ID: WP_003133769.1
• Coordinates: 5669271..5669576 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGV94_RS27080	5664971..5665552	+	582	WP_123903235.1	sce7726 family protein	-
EGV94_RS27085	5665539..5666609	-	1071	WP_023098928.1	beta family protein	-
EGV94_RS27090	5666599..5667435	-	837	WP_023098927.1	ImmA/IrrE family metallo-endopeptidase	-
EGV94_RS27095	5667428..5668102	-	675	WP_023098926.1	N-acetyltransferase	-
EGV94_RS27100	5668528..5668935	-	408	WP_049267485.1	hypothetical protein	-
EGV94_RS27105	5669271..5669576	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
EGV94_RS27110	5669588..5669866	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGV94_RS27120	5670195..5672423	+	2229	WP_003141628.1	TonB-dependent receptor	-
EGV94_RS27125	5672493..5673140	-	648	WP_003095021.1	carbonate dehydratase	-
EGV94_RS27130	5673202..5674440	-	1239	WP_003141631.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T114012 WP_003113526.1 NZ_CP033685:c5669866-5669588 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T114012 NZ_CP033685:c5669866-5669588 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT114012 WP_003133769.1 NZ_CP033685:c5669576-5669271 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT114012 NZ_CP033685:c5669576-5669271 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3