Detailed information of TA system TA114005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5992276..5992871
• Replicon: chromosome
• Accession: NZ_CP033684
• Organism: Pseudomonas aeruginosa strain H26027

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: EGV95_RS29035
• Protein ID: WP_003113526.1
• Coordinates: 5992593..5992871 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGV95_RS29030
• Protein ID: WP_003113527.1
• Coordinates: 5992276..5992581 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGV95_RS28995	5987415..5988263	+	849	WP_009875808.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
EGV95_RS29005	5988430..5989371	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
EGV95_RS29010	5989488..5990102	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
EGV95_RS29015	5990144..5990728	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
EGV95_RS29020	5990769..5991869	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
EGV95_RS29030	5992276..5992581	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
EGV95_RS29035	5992593..5992871	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGV95_RS29045	5993200..5995428	+	2229	WP_003113525.1	TonB-dependent receptor	-
EGV95_RS29050	5995498..5996145	-	648	WP_003095021.1	carbonate dehydratase	-
EGV95_RS29055	5996207..5997445	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T114005 WP_003113526.1 NZ_CP033684:c5992871-5992593 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T114005 NZ_CP033684:c5992871-5992593 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT114005 WP_003113527.1 NZ_CP033684:c5992581-5992276 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT114005 NZ_CP033684:c5992581-5992276 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3