Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2946116..2946891 Replicon chromosome
Accession NZ_CP033684
Organism Pseudomonas aeruginosa strain H26027

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag EGV95_RS14420 Protein ID WP_009518525.1
Coordinates 2946433..2946891 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7M2ZSF3
Locus tag EGV95_RS14415 Protein ID WP_023087286.1
Coordinates 2946116..2946433 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGV95_RS14385 2941440..2942090 - 651 WP_023087281.1 isoprenylcysteine carboxylmethyltransferase family protein -
EGV95_RS14390 2942087..2942326 - 240 WP_023087282.1 DUF2933 domain-containing protein -
EGV95_RS14395 2942338..2942739 - 402 WP_023087283.1 hypothetical protein -
EGV95_RS34370 2942951..2943476 + 526 Protein_2778 hypothetical protein -
EGV95_RS14405 2943473..2943895 + 423 WP_063837596.1 MerR family DNA-binding protein -
EGV95_RS14410 2943951..2945816 - 1866 WP_023087285.1 TraI domain-containing protein -
EGV95_RS14415 2946116..2946433 + 318 WP_023087286.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
EGV95_RS14420 2946433..2946891 + 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
EGV95_RS14425 2946918..2947277 + 360 WP_023087287.1 DUF3742 family protein -
EGV95_RS14430 2947284..2948807 - 1524 WP_023087288.1 conjugal transfer protein TraG N-terminal domain-containing protein -
EGV95_RS14435 2948821..2949180 - 360 WP_023087289.1 hypothetical protein -
EGV95_RS14440 2949177..2950571 - 1395 WP_023087290.1 integrating conjugative element protein -
EGV95_RS14445 2950581..2951528 - 948 WP_023087291.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2892820..3008036 115216


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T113999 WP_009518525.1 NZ_CP033684:2946433-2946891 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

>T113999 NZ_CP033684:2946433-2946891 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAACGATCCGGCGGGCTTCGAGTCCAACGCCAACGTCAAGCTCTTCCGGGCCTTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAATACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGACGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11156.69 Da        Isoelectric Point: 4.5685

>AT113999 WP_023087286.1 NZ_CP033684:2946116-2946433 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNLQGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp

>AT113999 NZ_CP033684:2946116-2946433 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGTTGACCTCCAAGGGCCAGATCACGCTGCCCAAGCCTATCCGGCAGGCGCTCGG
CGTTGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGCGTCGATGCCGAGCACGAGG
ATCCCGCCATCGGCGCGTTCCTGAGCCTGCTGGCCCGAGACATCGAAGCGGGTCGGAACCTCCAGGGCCTGCCCGAGGAT
CTGGCTCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB A0A7M2ZSF3

References