Detailed information of TA system TA113936

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50912..51182
• Replicon: plasmid pTB-nb3
• Accession: NZ_CP033634
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CQP61_RS02295
• Protein ID: WP_001312861.1
• Coordinates: 51024..51182 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	50912..50975 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS31385	45976..46230	+	255	WP_164473240.1	hypothetical protein	-
CQP61_RS02270	46701..47228	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
CQP61_RS02275	47284..47517	+	234	WP_021534894.1	DUF905 domain-containing protein	-
CQP61_RS02280	47581..49539	+	1959	WP_123891607.1	ParB/RepB/Spo0J family partition protein	-
CQP61_RS02285	49594..50028	+	435	WP_123891611.1	conjugation system SOS inhibitor PsiB	-
CQP61_RS02290	50025..50744	+	720	WP_086624612.1	plasmid SOS inhibition protein A	-
CQP61_RS31395	50756..50944	-	189	WP_001299721.1	hypothetical protein	-
-	50756..50980	+	225	NuclAT_0	-	-
-	50756..50980	+	225	NuclAT_0	-	-
-	50756..50980	+	225	NuclAT_0	-	-
-	50756..50980	+	225	NuclAT_0	-	-
-	50912..50975	-	64	-	-	Antitoxin
CQP61_RS02295	51024..51182	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CQP61_RS31675	51477..51803	+	327	WP_183129667.1	hypothetical protein	-
CQP61_RS02310	52123..52320	+	198	WP_045174704.1	hypothetical protein	-
CQP61_RS02315	52439..53260	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CQP61_RS02320	53557..54147	-	591	WP_165850686.1	transglycosylase SLT domain-containing protein	-
CQP61_RS02325	54490..54873	+	384	WP_001151565.1	relaxosome protein TraM	-
CQP61_RS02330	55067..55753	+	687	WP_072649631.1	PAS domain-containing protein	-
CQP61_RS02335	55847..56074	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82252	82252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T113936 WP_001312861.1 NZ_CP033634:51024-51182 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T113936 NZ_CP033634:51024-51182 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT113936 NZ_CP033634:c50975-50912 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin