Detailed information of TA system TA113932

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 10673..10927
• Replicon: plasmid pTB-nb3
• Accession: NZ_CP033634
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CQP61_RS02005
• Protein ID: WP_001362723.1
• Coordinates: 10721..10927 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	10673..10729 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS01965	6609..7355	+	747	WP_021534916.1	conjugal transfer pilus acetylase TraX	-
CQP61_RS01970	7410..7970	+	561	WP_000139341.1	fertility inhibition protein FinO	-
CQP61_RS01975	8101..8313	+	213	WP_032161968.1	hypothetical protein	-
CQP61_RS01980	8558..9019	+	462	WP_053287835.1	thermonuclease family protein	-
CQP61_RS01985	9065..9274	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
CQP61_RS01990	9312..9902	+	591	WP_053287836.1	DUF2726 domain-containing protein	-
CQP61_RS01995	10057..10506	+	450	WP_053287837.1	hypothetical protein	-
-	10673..10729	-	57	NuclAT_1	-	Antitoxin
-	10673..10729	-	57	NuclAT_1	-	Antitoxin
-	10673..10729	-	57	NuclAT_1	-	Antitoxin
-	10673..10729	-	57	NuclAT_1	-	Antitoxin
CQP61_RS02005	10721..10927	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
CQP61_RS02010	11211..11471	+	261	WP_021536205.1	replication regulatory protein RepA	-
CQP61_RS02020	11707..11781	+	75	WP_001365705.1	RepA leader peptide Tap	-
CQP61_RS02025	11774..12631	+	858	WP_021534913.1	incFII family plasmid replication initiator RepA	-
CQP61_RS02035	13331..13537	+	207	WP_121832032.1	hypothetical protein	-
CQP61_RS02040	13594..13929	+	336	WP_001147117.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CQP61_RS02045	13926..14276	+	351	WP_000127480.1	addiction module antitoxin	-
CQP61_RS02055	14995..15618	+	624	WP_021536204.1	hypothetical protein	-
CQP61_RS02060	15700..15879	-	180	Protein_17	PerC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82252	82252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T113932 WP_001362723.1 NZ_CP033634:10721-10927 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T113932 NZ_CP033634:10721-10927 [Escherichia coli]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT113932 NZ_CP033634:c10729-10673 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin