Detailed information of TA system TA113904

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26678..26947
• Replicon: plasmid unnamed4
• Accession: NZ_CP033629
• Organism: Klebsiella pneumoniae strain 4743

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DPQ49_RS30840
• Protein ID: WP_001312861.1
• Coordinates: 26789..26947 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26678..26743 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPQ49_RS29300	22388..22915	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
DPQ49_RS29305	22973..23206	+	234	WP_000006003.1	DUF905 family protein	-
DPQ49_RS29310	23267..25290	+	2024	Protein_32	ParB/RepB/Spo0J family partition protein	-
DPQ49_RS29315	25359..25793	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DPQ49_RS29320	25790..26509	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	26521..26745	+	225	NuclAT_0	-	-
-	26521..26745	+	225	NuclAT_0	-	-
-	26521..26745	+	225	NuclAT_0	-	-
-	26521..26745	+	225	NuclAT_0	-	-
-	26678..26743	+	66	-	-	Antitoxin
DPQ49_RS30840	26789..26947	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DPQ49_RS30995	27185..27562	-	378	Protein_36	hypothetical protein	-
DPQ49_RS29345	27862..28158	+	297	WP_001272251.1	hypothetical protein	-
DPQ49_RS29350	28269..29090	+	822	WP_001234445.1	DUF945 domain-containing protein	-
DPQ49_RS29355	29387..29989	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
DPQ49_RS29365	30312..30695	+	384	WP_001354030.1	relaxosome protein TraM	-
DPQ49_RS29370	30889..31560	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
DPQ49_RS29375	31697..31924	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-15	-	1..67867	67867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T113904 WP_001312861.1 NZ_CP033629:26789-26947 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T113904 NZ_CP033629:26789-26947 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT113904 NZ_CP033629:26678-26743 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin