Detailed information of TA system TA113897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4041888..4042618
• Replicon: chromosome
• Accession: NZ_CP033623
• Organism: Serratia marcescens strain N10A28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5C7D8E3
• Locus tag: EFZ62_RS19215
• Protein ID: WP_060424659.1
• Coordinates: 4041888..4042199 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EFZ62_RS19220
• Protein ID: WP_142110565.1
• Coordinates: 4042196..4042618 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFZ62_RS19185	4037199..4037924	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
EFZ62_RS19190	4037977..4039410	+	1434	WP_049198529.1	RNA polymerase factor sigma-54	-
EFZ62_RS19195	4039434..4039721	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
EFZ62_RS19200	4039877..4040338	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
EFZ62_RS19205	4040468..4041322	+	855	WP_015379177.1	RNase adapter RapZ	-
EFZ62_RS19210	4041319..4041591	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
EFZ62_RS19215	4041888..4042199	+	312	WP_060424659.1	type II toxin-antitoxin system HigB family toxin	Toxin
EFZ62_RS19220	4042196..4042618	+	423	WP_142110565.1	helix-turn-helix domain-containing protein	Antitoxin
EFZ62_RS19225	4042623..4044539	-	1917	WP_060424654.1	BglG family transcription antiterminator	-
EFZ62_RS19230	4044591..4045805	-	1215	WP_087763586.1	lactonase family protein	-
EFZ62_RS19235	4046143..4046883	-	741	WP_033648993.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T113897 WP_060424659.1 NZ_CP033623:4041888-4042199 [Serratia marcescens]
MHIISREPFNNATKQYPNEAAALEAIYKTLRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T113897 NZ_CP033623:4041888-4042199 [Serratia marcescens]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATACCCTAATGAAGCCGCTGCTCTCGAGGCCATTTA
TAAAACATTGCGACGCGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTAGGCGGCAACCACCTGCGAATTTTGTTCTTTGCCAGCTTTGAGACACAGAAAGTA
TTCATAAAGCACATTGTTAGCCACGCCGAGTACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15693.01 Da
• Isoelectric Point: 4.9650

>AT113897 WP_142110565.1 NZ_CP033623:4042196-4042618 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSGSQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQRQLSQSDFEHEIGKKSLVNQILSGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT113897 NZ_CP033623:4042196-4042618 [Serratia marcescens]
ATGATTACAGATGCCATTAAGGCCGCCGATGCGCTGCTGCGCGCCGTGCCCTTATTGCAGGGCAGCGGTTCTCAGCAAGA
CTACCGGGAAGCGCTCGAATTGGTGGAATATCTGCTGGAAAACGATGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGATAACAGCGCCGAGTTTACCGCATTCAACCAGCGTATCGCCCAACTCCCGCAGGGGGTGGCC
GCGCTTCGCGTATTGATGGAACAGCGCCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGCAAAAAGTCGTTGGTCAA
CCAAATCCTGAGCGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGCTTCAATCTGCCCCCGGCCC
TCTTTCTGCCGGAAGCAGATTAG

Structures

Toxin

• AlphaFold DB: A0A5C7D8E3