Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2741619..2742276 Replicon chromosome
Accession NZ_CP033623
Organism Serratia marcescens strain N10A28

Toxin (Protein)


Gene name higB Uniprot ID A0A0M4RY59
Locus tag EFZ62_RS13160 Protein ID WP_060420873.1
Coordinates 2741953..2742276 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0M5M3W3
Locus tag EFZ62_RS13155 Protein ID WP_060420390.1
Coordinates 2741619..2741915 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFZ62_RS13130 2737137..2737772 + 636 WP_063989218.1 flavodoxin family protein -
EFZ62_RS13135 2737916..2739031 + 1116 WP_060420396.1 glycosyltransferase family 9 protein -
EFZ62_RS13140 2738955..2740178 - 1224 WP_159318195.1 O-antigen ligase family protein -
EFZ62_RS13145 2740156..2741064 - 909 WP_159318197.1 sugar glycosyltransferase -
EFZ62_RS13150 2741369..2741611 + 243 WP_025303447.1 hypothetical protein -
EFZ62_RS13155 2741619..2741915 - 297 WP_060420390.1 helix-turn-helix domain-containing protein Antitoxin
EFZ62_RS13160 2741953..2742276 - 324 WP_060420873.1 toxin HigB-2 Toxin
EFZ62_RS13165 2742341..2742970 - 630 WP_159318966.1 alanyl-tRNA editing protein -
EFZ62_RS13170 2743056..2743991 + 936 WP_033647920.1 LysR family transcriptional regulator -
EFZ62_RS13175 2743974..2744621 - 648 WP_019452474.1 DNA oxidative demethylase AlkB -
EFZ62_RS13180 2744761..2745639 + 879 WP_087763156.1 EamA family transporter RarD -
EFZ62_RS13185 2745641..2746399 - 759 WP_072271000.1 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-83)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12370.15 Da        Isoelectric Point: 8.8132

>T113893 WP_060420873.1 NZ_CP033623:c2742276-2741953 [Serratia marcescens]
MDYLEFIETTVFSRKRAELMKDDDFQAFQSYLLLNHHEGDTISHTGGCKKIRWGLDGKGKRGGVRVIYYLTAPSGRVYLL
LIYRKNEKDDLSGQEKAILRSIVQQLD

Download         Length: 324 bp

>T113893 NZ_CP033623:c2742276-2741953 [Serratia marcescens]
ATGGACTATCTGGAGTTTATCGAAACGACCGTTTTCAGCCGAAAGCGGGCTGAATTGATGAAGGATGATGACTTTCAGGC
TTTCCAAAGCTATTTGCTGTTGAACCATCATGAAGGGGATACGATCAGCCACACGGGCGGATGCAAAAAGATACGCTGGG
GGCTGGACGGAAAGGGAAAACGCGGCGGCGTAAGAGTGATTTATTATCTGACGGCGCCCAGCGGCCGAGTCTATTTATTG
TTGATATACCGTAAAAATGAAAAGGATGATTTATCCGGACAGGAAAAGGCGATATTGAGATCCATCGTTCAACAACTTGA
TTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11003.00 Da        Isoelectric Point: 10.1906

>AT113893 WP_060420390.1 NZ_CP033623:c2741915-2741619 [Serratia marcescens]
MKKELFDDLVSSMNEMVAIEKGKMTPKSEHVHRHAIPDVKLLRKSRGMKQAEFAKAVGASVGLVQSWEQERRIPSGSALK
LLLLIERNPALLDELLVI

Download         Length: 297 bp

>AT113893 NZ_CP033623:c2741915-2741619 [Serratia marcescens]
ATGAAAAAAGAACTTTTCGACGATTTGGTCAGCAGTATGAACGAAATGGTGGCGATCGAAAAGGGTAAGATGACGCCAAA
ATCCGAGCATGTTCACCGCCATGCTATTCCAGACGTCAAGCTGCTGCGCAAATCCCGTGGGATGAAGCAGGCGGAGTTTG
CCAAGGCTGTGGGGGCCAGCGTGGGACTGGTGCAAAGCTGGGAGCAGGAACGCCGCATACCCTCGGGGTCGGCACTGAAA
CTTCTGCTGCTGATTGAGCGTAACCCGGCATTGCTCGATGAGTTGCTGGTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4RY59

Antitoxin

Source ID Structure
AlphaFold DB A0A0M5M3W3

References