Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 292544..293238 Replicon chromosome
Accession NZ_CP033605
Organism Escherichia coli O157:H7 strain TR01

Toxin (Protein)


Gene name yafO Uniprot ID A0A080JBV5
Locus tag EEL45_RS01325 Protein ID WP_032280310.1
Coordinates 292840..293238 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EEL45_RS01320 Protein ID WP_000554758.1
Coordinates 292544..292837 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL45_RS01300 288253..288750 + 498 Protein_252 REP-associated tyrosine transposase RayT -
EEL45_RS01305 288897..290609 - 1713 Protein_253 flagellar biosynthesis protein FlhA -
EEL45_RS01310 290581..291366 + 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
EEL45_RS01315 291437..292492 + 1056 WP_001226183.1 DNA polymerase IV -
EEL45_RS01320 292544..292837 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EEL45_RS01325 292840..293238 + 399 WP_032280310.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EEL45_RS01330 293248..293700 + 453 WP_001059871.1 GNAT family N-acetyltransferase -
EEL45_RS01335 294132..294743 + 612 WP_192892690.1 peptide chain release factor H -
EEL45_RS01340 294800..296257 - 1458 WP_001293010.1 cytosol nonspecific dipeptidase -
EEL45_RS01345 296518..296976 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 297572..297652 + 81 NuclAT_9 - -
- 297572..297652 + 81 NuclAT_9 - -
- 297572..297652 + 81 NuclAT_9 - -
- 297572..297652 + 81 NuclAT_9 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 272297..293700 21403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15501.91 Da        Isoelectric Point: 8.0949

>T113836 WP_032280310.1 NZ_CP033605:292840-293238 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTVDFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T113836 NZ_CP033605:292840-293238 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGTGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT113836 WP_000554758.1 NZ_CP033605:292544-292837 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT113836 NZ_CP033605:292544-292837 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCTGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A080JBV5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References