Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 286577..287118 Replicon chromosome
Accession NZ_CP033605
Organism Escherichia coli O157:H7 strain TR01

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag EEL45_RS01285 Protein ID WP_000615979.1
Coordinates 286577..286855 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EEL45_RS01290 Protein ID WP_000729704.1
Coordinates 286858..287118 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL45_RS01270 284159..284737 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EEL45_RS01275 284943..285710 + 768 WP_000333379.1 class II glutamine amidotransferase -
EEL45_RS01280 285681..286421 - 741 WP_001225679.1 murein L,D-transpeptidase -
EEL45_RS01285 286577..286855 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EEL45_RS01290 286858..287118 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EEL45_RS01295 287328..288077 + 750 WP_032280313.1 C40 family peptidase -
EEL45_RS01300 288253..288750 + 498 Protein_252 REP-associated tyrosine transposase RayT -
EEL45_RS01305 288897..290609 - 1713 Protein_253 flagellar biosynthesis protein FlhA -
EEL45_RS01310 290581..291366 + 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 272297..293700 21403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T113835 WP_000615979.1 NZ_CP033605:c286855-286577 [Escherichia coli O157:H7]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T113835 NZ_CP033605:c286855-286577 [Escherichia coli O157:H7]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT113835 WP_000729704.1 NZ_CP033605:c287118-286858 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT113835 NZ_CP033605:c287118-286858 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References