Detailed information of TA system TA113815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 140113..140844
• Replicon: plasmid pKC-Na-R1
• Accession: NZ_CP033574
• Organism: Shewanella algae strain KC-Na-R1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EEY24_RS00805
• Protein ID: WP_086904557.1
• Coordinates: 140533..140844 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EEY24_RS00800
• Protein ID: WP_086904558.1
• Coordinates: 140113..140529 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEY24_RS00735	135267..135713	-	447	WP_123115314.1	hypothetical protein	-
EEY24_RS00740	135717..136013	-	297	WP_123115315.1	hypothetical protein	-
EEY24_RS00745	136034..136294	-	261	WP_123115316.1	hypothetical protein	-
EEY24_RS00750	136279..136509	-	231	WP_123115317.1	hypothetical protein	-
EEY24_RS00755	136534..136872	-	339	WP_123115318.1	hypothetical protein	-
EEY24_RS00760	136923..137480	-	558	WP_123115319.1	hypothetical protein	-
EEY24_RS00765	137499..137747	-	249	WP_086904566.1	DUF3850 domain-containing protein	-
EEY24_RS23620	137766..138176	-	411	WP_164489352.1	hypothetical protein	-
EEY24_RS00775	138189..138434	-	246	WP_086904564.1	antirestriction protein ArdR	-
EEY24_RS00780	138431..138637	-	207	WP_086904563.1	hypothetical protein	-
EEY24_RS00785	138905..139192	-	288	WP_086904561.1	hypothetical protein	-
EEY24_RS00795	139659..139958	+	300	WP_123115321.1	hypothetical protein	-
EEY24_RS00800	140113..140529	-	417	WP_086904558.1	transcriptional regulator	Antitoxin
EEY24_RS00805	140533..140844	-	312	WP_086904557.1	type II toxin-antitoxin system HigB family toxin	Toxin
EEY24_RS00810	141014..141589	-	576	WP_086904556.1	SOS response-associated peptidase	-
EEY24_RS00815	141761..142183	-	423	WP_086904555.1	hypothetical protein	-
EEY24_RS00820	142225..142545	-	321	WP_123115322.1	hypothetical protein	-
EEY24_RS00825	142588..143250	-	663	WP_123115323.1	site-specific integrase	-
EEY24_RS00830	143403..145280	-	1878	WP_086903395.1	ATP-dependent helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrA1 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ant(2'')-Ia / blaVEB-1	-	1..167144	167144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Antitoxin HigA (3-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12475.11 Da
• Isoelectric Point: 9.8965

>T113815 WP_086904557.1 NZ_CP033574:c140844-140533 [Shewanella algae]
VHVLSRKPFNDAARHYPNQREALVSAYQTLRAGEFRTPNELKQVFPSLDNFKYRDKWWVIDIGGNHLRLIAFIEFRDNRM
YVKHIVPHAEYDDLTDRYRRNKE

Nucleotide

• Length: 312 bp

>T113815 NZ_CP033574:c140844-140533 [Shewanella algae]
GTGCATGTACTCTCTCGAAAACCATTTAATGATGCTGCCAGACACTACCCAAATCAGCGAGAAGCGCTAGTTAGCGCCTA
CCAGACATTGAGGGCCGGCGAGTTCAGAACGCCAAATGAACTCAAGCAAGTGTTCCCAAGTTTGGATAACTTCAAGTATC
GAGATAAATGGTGGGTAATAGACATTGGAGGCAATCATCTGAGGCTTATTGCCTTTATTGAATTCCGGGACAACCGAATG
TATGTCAAACATATAGTGCCACACGCAGAATATGATGATCTAACTGACAGGTACAGGCGAAATAAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15591.69 Da
• Isoelectric Point: 4.4403

>AT113815 WP_086904558.1 NZ_CP033574:c140529-140113 [Shewanella algae]
MFPQACHLIREALEMAPFIAEINGQDDYDKALATMDTLIDDYEANKHVINMLSITIERWESSADEFAQFNQAVEEADTGI
ALLKTLMDQYQLGVNDLPELGTKANVSKLLNHTDGKKLTRNHIEALSKRFNLSPAMFF

Nucleotide

• Length: 417 bp

>AT113815 NZ_CP033574:c140529-140113 [Shewanella algae]
ATGTTTCCTCAAGCATGCCACTTAATCAGAGAAGCCTTGGAAATGGCGCCGTTCATCGCTGAGATCAATGGCCAGGACGA
CTATGACAAGGCGCTTGCCACCATGGACACCTTGATCGATGATTACGAGGCAAATAAACACGTTATCAACATGCTTTCAA
TAACCATTGAACGCTGGGAAAGTAGTGCCGATGAATTTGCCCAATTTAACCAAGCTGTCGAGGAAGCAGACACCGGCATC
GCCTTGCTGAAAACGCTGATGGATCAATATCAACTTGGCGTGAACGATTTACCAGAGCTTGGTACCAAAGCCAATGTCAG
CAAGTTGCTTAACCACACCGATGGCAAGAAGCTTACCAGAAACCACATCGAGGCCTTGAGCAAGCGCTTCAACCTCTCGC
CAGCAATGTTTTTCTAA