Detailed information of TA system TA113786

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4379907..4380132
• Replicon: chromosome
• Accession: NZ_CP033510
• Organism: Shigella flexneri strain 2016AM-0877

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: EEL47_RS22255
• Protein ID: WP_000813254.1
• Coordinates: 4379977..4380132 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4379907..4379965 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL47_RS26465	4375905..4376074	+	170	Protein_4238	hypothetical protein	-
EEL47_RS22220	4376220..4376966	+	747	WP_000788996.1	ATP-binding protein	-
EEL47_RS22225	4376981..4377403	+	423	WP_001118168.1	DUF977 family protein	-
EEL47_RS22230	4377461..4377817	+	357	WP_005048249.1	hypothetical protein	-
EEL47_RS22235	4377910..4378128	+	219	WP_000256998.1	DUF4014 family protein	-
EEL47_RS22240	4378130..4378495	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
EEL47_RS22245	4378492..4379157	+	666	WP_000208062.1	hypothetical protein	-
EEL47_RS22250	4379157..4379522	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	4379907..4379965	-	59	-	-	Antitoxin
EEL47_RS22255	4379977..4380132	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
EEL47_RS22270	4381469..4382068	+	600	WP_000940329.1	DUF1367 family protein	-
EEL47_RS22275	4382068..4382358	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
EEL47_RS22280	4382355..4382909	+	555	WP_000640143.1	DUF1133 family protein	-
EEL47_RS22285	4383062..4383235	+	174	WP_000504450.1	hypothetical protein	-
EEL47_RS22290	4383297..4384436	+	1140	WP_000088354.1	IS3 family transposase	-
EEL47_RS22295	4384507..4384989	+	483	WP_000068107.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	4371990..4436136	64146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T113786 WP_000813254.1 NZ_CP033510:4379977-4380132 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T113786 NZ_CP033510:4379977-4380132 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT113786 NZ_CP033510:c4379965-4379907 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin