Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3365123..3365741 Replicon chromosome
Accession NZ_CP033510
Organism Shigella flexneri strain 2016AM-0877

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EEL47_RS16950 Protein ID WP_001291435.1
Coordinates 3365123..3365341 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag EEL47_RS16955 Protein ID WP_000344797.1
Coordinates 3365367..3365741 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL47_RS16920 (3361015) 3361015..3361326 - 312 WP_000409911.1 MGMT family protein -
EEL47_RS16930 (3361705) 3361705..3362058 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EEL47_RS16935 (3362100) 3362100..3363650 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EEL47_RS16940 (3363814) 3363814..3364284 - 471 WP_000136192.1 YlaC family protein -
EEL47_RS16945 (3364400) 3364400..3364951 - 552 Protein_3226 maltose O-acetyltransferase -
EEL47_RS16950 (3365123) 3365123..3365341 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EEL47_RS16955 (3365367) 3365367..3365741 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
EEL47_RS16960 (3366287) 3366287..3369436 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
EEL47_RS16965 (3369459) 3369459..3370652 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3359657..3360985 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T113784 WP_001291435.1 NZ_CP033510:c3365341-3365123 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T113784 NZ_CP033510:c3365341-3365123 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT113784 WP_000344797.1 NZ_CP033510:c3365741-3365367 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT113784 NZ_CP033510:c3365741-3365367 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References