Detailed information of TA system TA113783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3327941..3328785
• Replicon: chromosome
• Accession: NZ_CP033510
• Organism: Shigella flexneri strain 2016AM-0877

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: EEL47_RS16750
• Protein ID: WP_014532172.1
• Coordinates: 3327941..3328402 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: EEL47_RS16760
• Protein ID: WP_005053053.1
• Coordinates: 3328474..3328785 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL47_RS16735 (3323109)	3323109..3324839	-	1731	WP_000645013.1	hypothetical protein	-
EEL47_RS16740 (3324892)	3324892..3327045	-	2154	WP_005060811.1	SEL1-like repeat protein	-
EEL47_RS16745 (3327196)	3327196..3327893	+	698	WP_252965753.1	IS1 family transposase	-
EEL47_RS16750 (3327941)	3327941..3328402	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
EEL47_RS16760 (3328474)	3328474..3328785	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
EEL47_RS16765 (3328969)	3328969..3329859	-	891	WP_000971346.1	heme o synthase	-
EEL47_RS16770 (3329871)	3329871..3330200	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EEL47_RS16775 (3330200)	3330200..3330814	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EEL47_RS16780 (3330804)	3330804..3332795	-	1992	Protein_3194	cytochrome o ubiquinol oxidase subunit I	-
EEL47_RS16785 (3332817)	3332817..3333314	-	498	Protein_3195	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.007	flank	IS/Tn	-	-	3327390..3327893	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T113783 WP_014532172.1 NZ_CP033510:c3328402-3327941 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T113783 NZ_CP033510:c3328402-3327941 [Shigella flexneri]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT113783 WP_005053053.1 NZ_CP033510:c3328785-3328474 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT113783 NZ_CP033510:c3328785-3328474 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0