Detailed information of TA system TA113781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3173261..3173955
• Replicon: chromosome
• Accession: NZ_CP033510
• Organism: Shigella flexneri strain 2016AM-0877

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: EEL47_RS15955
• Protein ID: WP_001263491.1
• Coordinates: 3173557..3173955 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: Q0T7Q4
• Locus tag: EEL47_RS15950
• Protein ID: WP_000554759.1
• Coordinates: 3173261..3173554 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL47_RS15930 (3168900)	3168900..3169397	+	498	WP_000006251.1	REP-associated tyrosine transposase RayT	-
EEL47_RS15935 (3169614)	3169614..3171326	-	1713	Protein_3038	flagellar biosynthesis protein FlhA	-
EEL47_RS15940 (3171298)	3171298..3172083	+	786	WP_000207560.1	putative lateral flagellar export/assembly protein LafU	-
EEL47_RS15945 (3172154)	3172154..3173209	+	1056	WP_001226172.1	DNA polymerase IV	-
EEL47_RS15950 (3173261)	3173261..3173554	+	294	WP_000554759.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EEL47_RS15955 (3173557)	3173557..3173955	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EEL47_RS15960 (3173965)	3173965..3174417	+	453	WP_001059860.1	GNAT family N-acetyltransferase	-
EEL47_RS15965 (3174663)	3174663..3175706	+	1044	WP_005053205.1	RNA ligase RtcB family protein	-
EEL47_RS15970 (3175822)	3175822..3176382	+	561	Protein_3045	peptide chain release factor H	-
EEL47_RS15975 (3176439)	3176439..3177896	-	1458	WP_001293030.1	cytosol nonspecific dipeptidase	-
EEL47_RS15980 (3178158)	3178158..3178616	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3095116..3175706	80590

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T113781 WP_001263491.1 NZ_CP033510:3173557-3173955 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T113781 NZ_CP033510:3173557-3173955 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11232.82 Da
• Isoelectric Point: 7.0153

>AT113781 WP_000554759.1 NZ_CP033510:3173261-3173554 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT113781 NZ_CP033510:3173261-3173554 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A4P7TR46