Detailed information of TA system TA113775

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 323259..323484
• Replicon: chromosome
• Accession: NZ_CP033510
• Organism: Shigella flexneri strain 2016AM-0877

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: EEL47_RS01855
• Protein ID: WP_000813254.1
• Coordinates: 323259..323414 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	323426..323484 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL47_RS01795	318678..319028	+	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-
EEL47_RS01800	319025..319522	+	498	Protein_339	IS66 family transposase zinc-finger binding domain-containing protein	-
EEL47_RS01805	319578..320275	+	698	WP_223368647.1	IS1 family transposase	-
EEL47_RS01830	320702..321390	-	689	Protein_341	bacteriophage antitermination protein Q	-
EEL47_RS01835	321387..321752	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
EEL47_RS01840	321753..322811	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
EEL47_RS01845	322813..323091	-	279	WP_011069426.1	hypothetical protein	-
EEL47_RS01855	323259..323414	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	323426..323484	+	59	-	-	Antitoxin
EEL47_RS01870	324066..324482	-	417	WP_005069274.1	hypothetical protein	-
EEL47_RS01875	324509..324649	+	141	Protein_347	DUF4224 domain-containing protein	-
EEL47_RS01880	324649..325699	+	1051	Protein_348	tyrosine-type recombinase/integrase	-
EEL47_RS01890	325910..326707	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
EEL47_RS01900	327045..328307	+	1263	Protein_350	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	312019..360044	48025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T113775 WP_000813254.1 NZ_CP033510:c323414-323259 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T113775 NZ_CP033510:c323414-323259 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT113775 NZ_CP033510:323426-323484 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin