Detailed information of TA system TA113760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3884540..3885270
• Replicon: chromosome
• Accession: NZ_CP033504
• Organism: Serratia sp. LS-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: EDY99_RS18515
• Protein ID: WP_004937033.1
• Coordinates: 3884540..3884851 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EDY99_RS18520
• Protein ID: WP_033644489.1
• Coordinates: 3884848..3885270 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDY99_RS18485	3879855..3880580	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
EDY99_RS18490	3880633..3882066	+	1434	WP_004937039.1	RNA polymerase factor sigma-54	-
EDY99_RS18495	3882090..3882377	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
EDY99_RS18500	3882533..3882994	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
EDY99_RS18505	3883122..3883976	+	855	WP_015379177.1	RNase adapter RapZ	-
EDY99_RS18510	3883973..3884245	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
EDY99_RS18515	3884540..3884851	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
EDY99_RS18520	3884848..3885270	+	423	WP_033644489.1	helix-turn-helix domain-containing protein	Antitoxin
EDY99_RS18525	3885277..3887193	-	1917	WP_033644486.1	BglG family transcription antiterminator	-
EDY99_RS18530	3887245..3888459	-	1215	WP_123061988.1	lactonase family protein	-
EDY99_RS18535	3888547..3889287	-	741	WP_046687877.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T113760 WP_004937033.1 NZ_CP033504:3884540-3884851 [Serratia sp. LS-1]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T113760 NZ_CP033504:3884540-3884851 [Serratia sp. LS-1]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAGTACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCGTCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT113760 WP_033644489.1 NZ_CP033504:3884848-3885270 [Serratia sp. LS-1]
MITDAIKAADALLRAVPLLQGSHAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT113760 NZ_CP033504:3884848-3885270 [Serratia sp. LS-1]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTACCCTTATTGCAGGGCAGCCACGCTCAGCAAGA
CTACCGAGAAGCGCTCGAATTGGTGGAATATCTGCTGGAAAACGATGAACGCCATCCGCTGATTGACATGTTGGCAAAAA
AAATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAACTCCCGCAGGGGGTGGCC
GCGCTTCGCGTATTGATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGCAAAAAGTCGTTGGTCAA
CCAAATCCTGAACGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGCTTCAATCTGCCCCCAGCCC
TGTTCCTGCCGGAAGCGGATTAA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63