Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3826789..3827417 Replicon chromosome
Accession NZ_CP033504
Organism Serratia sp. LS-1

Toxin (Protein)


Gene name higB Uniprot ID A0A8B4GSN3
Locus tag EDY99_RS18205 Protein ID WP_042785415.1
Coordinates 3826789..3827079 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B4GQY7
Locus tag EDY99_RS18210 Protein ID WP_015379150.1
Coordinates 3827094..3827417 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDY99_RS18195 3823362..3825383 + 2022 WP_122016772.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
EDY99_RS18200 3825418..3826557 - 1140 WP_122016771.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
EDY99_RS18205 3826789..3827079 + 291 WP_042785415.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EDY99_RS18210 3827094..3827417 + 324 WP_015379150.1 HigA family addiction module antidote protein Antitoxin
EDY99_RS18215 3827410..3827922 + 513 WP_042785417.1 M48 family metallopeptidase -
EDY99_RS18220 3827973..3828953 + 981 WP_046897262.1 Gfo/Idh/MocA family oxidoreductase -
EDY99_RS18225 3828955..3829686 + 732 WP_096242615.1 glutamine amidotransferase -
EDY99_RS18230 3829942..3830907 + 966 WP_164489293.1 TerC family protein -
EDY99_RS18235 3831171..3832406 + 1236 WP_028128305.1 serine/threonine transporter SstT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-94)

Antitoxin

Cro/C1-type helix-turn-helix domain

(24-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11672.37 Da        Isoelectric Point: 10.4297

>T113759 WP_042785415.1 NZ_CP033504:3826789-3827079 [Serratia sp. LS-1]
MIKSFRDRYLEQFYLEGRRSRLIPGALERQLARKLDMLAAAQQERDLHHPKGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Download         Length: 291 bp

>T113759 NZ_CP033504:3826789-3827079 [Serratia sp. LS-1]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGAAGGCAGGCGCAGCCGTCTTATCCCCGGCGCGCT
CGAGCGGCAGTTGGCCAGAAAGCTCGATATGTTAGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCAAGGGTAACT
ATTACAAGCGGCTATCCGGGCCATTTCAGGGATGGTCGAGTCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCCGAAAAAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12141.78 Da        Isoelectric Point: 6.2285

>AT113759 WP_015379150.1 NZ_CP033504:3827094-3827417 [Serratia sp. LS-1]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Download         Length: 324 bp

>AT113759 NZ_CP033504:3827094-3827417 [Serratia sp. LS-1]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGAGAAGTGCTGCAGGAAGAATATTTAACGCCGTTAGGCATCGG
CCAAGAAGAACTGGCAAAGGTATTGGGCGTCACCCGCGCCACCGTCAATCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAATCTGTTTGCCGATCTGTTCGAAACGACCGCCGACTTTTGGCTAAACCTGCAGGCGGCACACGATCGC
TGGGAAGCCAGACAGGCCGATGCGCAGCGTGTGCGGCTGCGGCCAATTAAAGAAATACTGGGACGCAATATCACTCATGC
ATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4GSN3

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GQY7

References