Detailed information of TA system TA113743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 45383..45884
• Replicon: plasmid p3442-FRI-1
• Accession: NZ_CP033467
• Organism: Enterobacter cloacae strain E3442

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EEI76_RS22015
• Protein ID: WP_123061045.1
• Coordinates: 45609..45884 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: EEI76_RS22010
• Protein ID: WP_103061436.1
• Coordinates: 45383..45628 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEI76_RS21975	40455..40718	+	264	WP_123061038.1	hypothetical protein	-
EEI76_RS21980	40761..41591	+	831	WP_123061039.1	type I restriction-modification system subunit M	-
EEI76_RS21985	42218..42748	+	531	WP_123061040.1	antirestriction protein	-
EEI76_RS21990	42760..43215	-	456	WP_123061041.1	transglycosylase SLT domain-containing protein	-
EEI76_RS21995	43682..44056	+	375	WP_123061042.1	relaxosome protein TraM	-
EEI76_RS22005	44896..45141	-	246	WP_123061044.1	hypothetical protein	-
EEI76_RS22010	45383..45628	+	246	WP_103061436.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EEI76_RS22015	45609..45884	+	276	WP_123061045.1	Txe/YoeB family addiction module toxin	Toxin
EEI76_RS22020	45997..46203	+	207	WP_123061046.1	hypothetical protein	-
EEI76_RS22025	46200..46517	+	318	WP_123061047.1	hypothetical protein	-
EEI76_RS22030	46591..47407	-	817	Protein_55	IS3 family transposase	-
EEI76_RS22035	47479..48648	-	1170	Protein_56	IS256 family transposase	-
EEI76_RS22040	48989..49249	-	261	WP_164472304.1	hypothetical protein	-
EEI76_RS22045	49349..49794	-	446	Protein_58	transposase	-
EEI76_RS22050	50384..50884	+	501	WP_123061084.1	Ail/Lom family outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaFRI-5	-	1..93658	93658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10891.43 Da
• Isoelectric Point: 10.4471

>T113743 WP_123061045.1 NZ_CP033467:45609-45884 [Enterobacter cloacae]
MSKWNIKFTRNGADDLSYWRDTDKKKFERIRQLLRSIEADPLTGIGKPERLRHHKDPALYSRRIDQSHRLVYSIKSGEII
IYAARYHYGDK

Nucleotide

• Length: 276 bp

>T113743 NZ_CP033467:45609-45884 [Enterobacter cloacae]
TTGTCGAAGTGGAATATTAAGTTCACCAGGAACGGAGCTGATGACCTTAGCTACTGGCGTGATACTGACAAAAAGAAGTT
TGAGCGTATCCGGCAGCTGTTGCGCAGCATCGAGGCTGATCCGCTGACAGGTATCGGAAAACCCGAACGGCTGCGGCACC
ACAAAGATCCGGCGCTCTATTCCCGCCGGATTGATCAGAGTCACCGTCTGGTCTATTCAATCAAGAGCGGAGAAATCATC
ATCTACGCAGCCCGTTATCACTATGGTGACAAATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9289.43 Da
• Isoelectric Point: 4.5069

>AT113743 WP_103061436.1 NZ_CP033467:45383-45628 [Enterobacter cloacae]
MHAINFTDARKHFADTMKRVTDDAEPVRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAAHINESMKQAERGEFVEVE
Y

Nucleotide

• Length: 246 bp

>AT113743 NZ_CP033467:45383-45628 [Enterobacter cloacae]
ATGCATGCAATCAATTTTACTGATGCCCGTAAACATTTTGCTGACACCATGAAGCGCGTCACTGATGATGCTGAACCTGT
ACGCGTCATGCGTCGTGACGCACCTGATATTGTTATGATCGATGCAGGAGAGTATGAGGCGCTAATCGAAACGGTCTATC
TGTTCAGCAATCCGGCGAACGCCGCGCATATCAATGAGTCAATGAAACAGGCGGAGCGCGGCGAATTTGTCGAAGTGGAA
TATTAA