Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4361423..4362080 Replicon chromosome
Accession NZ_CP033466
Organism Enterobacter cloacae strain E3442

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag EEI76_RS21145 Protein ID WP_015571638.1
Coordinates 4361781..4362080 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0F0ZCT3
Locus tag EEI76_RS21140 Protein ID WP_022651701.1
Coordinates 4361423..4361770 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEI76_RS21115 4356757..4357755 + 999 WP_045346167.1 transketolase family protein -
EEI76_RS21120 4357830..4358561 + 732 WP_123060950.1 class I SAM-dependent methyltransferase -
EEI76_RS21125 4358558..4359160 - 603 WP_123060951.1 short chain dehydrogenase -
EEI76_RS21130 4359258..4360145 + 888 WP_032660046.1 LysR family transcriptional regulator -
EEI76_RS21135 4360166..4361347 + 1182 WP_123060952.1 PLP-dependent aminotransferase family protein -
EEI76_RS21140 4361423..4361770 - 348 WP_022651701.1 HigA family addiction module antidote protein Antitoxin
EEI76_RS21145 4361781..4362080 - 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EEI76_RS21150 4362331..4363491 - 1161 WP_086581339.1 HlyD family efflux transporter periplasmic adaptor subunit -
EEI76_RS21155 4363511..4365703 - 2193 WP_022651699.1 type I secretion system permease/ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T113738 WP_015571638.1 NZ_CP033466:c4362080-4361781 [Enterobacter cloacae]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T113738 NZ_CP033466:c4362080-4361781 [Enterobacter cloacae]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCTATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATACTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13096.09 Da        Isoelectric Point: 9.5932

>AT113738 WP_022651701.1 NZ_CP033466:c4361770-4361423 [Enterobacter cloacae]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVA

Download         Length: 348 bp

>AT113738 NZ_CP033466:c4361770-4361423 [Enterobacter cloacae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCGGACGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F0ZCT3

References