Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3235790..3236520 Replicon chromosome
Accession NZ_CP033466
Organism Enterobacter cloacae strain E3442

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EEI76_RS15625 Protein ID WP_123060833.1
Coordinates 3236206..3236520 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EEI76_RS15620 Protein ID WP_023323924.1
Coordinates 3235790..3236209 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEI76_RS15600 3231518..3232862 - 1345 Protein_2995 two-component system sensor histidine kinase UhpB -
EEI76_RS15610 3233836..3235438 - 1603 Protein_2996 acetolactate synthase large subunit -
EEI76_RS15615 3235545..3235643 - 99 WP_057979964.1 ilvB operon leader peptide IvbL -
EEI76_RS15620 3235790..3236209 - 420 WP_023323924.1 helix-turn-helix domain-containing protein Antitoxin
EEI76_RS15625 3236206..3236520 - 315 WP_123060833.1 type II toxin-antitoxin system HigB family toxin Toxin
EEI76_RS15630 3237007..3237096 + 90 WP_017383683.1 type I toxin-antitoxin system toxin TisB -
EEI76_RS15635 3237161..3237607 + 447 WP_003861064.1 GNAT family N-acetyltransferase -
EEI76_RS15640 3237670..3238503 + 834 WP_023323922.1 DMT family transporter -
EEI76_RS15645 3238679..3239863 + 1185 WP_003861070.1 multidrug efflux MFS transporter EmrD -
EEI76_RS15650 3239769..3240854 - 1086 Protein_3004 cellulase family glycosylhydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(1-139)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12430.61 Da        Isoelectric Point: 10.2825

>T113736 WP_123060833.1 NZ_CP033466:c3236520-3236206 [Enterobacter cloacae]
MHLISMKAILDAVSQFLQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNILRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T113736 NZ_CP033466:c3236520-3236206 [Enterobacter cloacae]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCTGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACATTCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15499.15 Da        Isoelectric Point: 5.7670

>AT113736 WP_023323924.1 NZ_CP033466:c3236209-3235790 [Enterobacter cloacae]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT113736 NZ_CP033466:c3236209-3235790 [Enterobacter cloacae]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGT
GCGCCAGAATTAGCCGTTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References