113706

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 909555..910150 Replicon chromosome
Accession NZ_CP033439
Organism Pseudomonas aeruginosa strain SP4528

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag D8668_RS04120 Protein ID WP_003113526.1
Coordinates 909555..909833 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag D8668_RS04125 Protein ID WP_003111575.1
Coordinates 909845..910150 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8668_RS04100 904980..906218 + 1239 WP_031686604.1 C69 family dipeptidase -
D8668_RS04105 906280..906927 + 648 WP_003095021.1 carbonate dehydratase -
D8668_RS04110 906998..909226 - 2229 WP_033991161.1 TonB-dependent receptor -
D8668_RS04120 909555..909833 + 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8668_RS04125 909845..910150 + 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
D8668_RS04135 910556..911656 - 1101 WP_003099270.1 redox-regulated ATPase YchF -
D8668_RS04140 911697..912281 - 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
D8668_RS04145 912323..912937 - 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
D8668_RS04150 913054..913995 - 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
D8668_RS04160 914162..915010 - 849 WP_031686602.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T113706 WP_003113526.1 NZ_CP033439:909555-909833 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T113706 NZ_CP033439:909555-909833 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGACGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT113706 WP_003111575.1 NZ_CP033439:909845-910150 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT113706 NZ_CP033439:909845-910150 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References