Detailed information of TA system TA113696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1803789..1804444
• Replicon: chromosome
• Accession: NZ_CP033430
• Organism: Burkholderia gladioli strain Burkholderia gladioli Co14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EDD84_RS09235
• Protein ID: WP_123037133.1
• Coordinates: 1804100..1804444 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EDD84_RS09230
• Protein ID: WP_123036378.1
• Coordinates: 1803789..1804103 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDD84_RS09195	1798824..1799537	+	714	WP_036034312.1	acetyltransferase	-
EDD84_RS36660	1799715..1799882	-	168	WP_155301612.1	hypothetical protein	-
EDD84_RS09205	1800101..1800784	+	684	WP_123036375.1	hypothetical protein	-
EDD84_RS09210	1800845..1801471	+	627	WP_123036376.1	cytochrome b/b6 domain-containing protein	-
EDD84_RS09215	1801471..1802226	+	756	WP_036034318.1	molybdopterin-dependent oxidoreductase	-
EDD84_RS09220	1802306..1802743	-	438	WP_123036377.1	hypothetical protein	-
EDD84_RS09225	1802939..1803658	+	720	WP_036034321.1	DUF1223 domain-containing protein	-
EDD84_RS09230	1803789..1804103	-	315	WP_123036378.1	transcriptional regulator	Antitoxin
EDD84_RS09235	1804100..1804444	-	345	WP_123037133.1	toxin	Toxin
EDD84_RS36665	1804647..1805243	+	597	WP_036034324.1	hypothetical protein	-
EDD84_RS09245	1805420..1806040	-	621	WP_036034325.1	riboflavin synthase	-
EDD84_RS09250	1806063..1807181	-	1119	WP_036034326.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
EDD84_RS09255	1807203..1808489	-	1287	WP_060006052.1	glutamate-1-semialdehyde 2,1-aminomutase	-
EDD84_RS09260	1808685..1809140	-	456	WP_013696909.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-90)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13372.22 Da
• Isoelectric Point: 9.7511

>T113696 WP_123037133.1 NZ_CP033430:c1804444-1804100 [Burkholderia gladioli]
VQATFIELPPFSRERENYLADEEYAVPQKALLANPEAGDLIKGAGSLRKLRFADKRRQKGKRGGLRVIYYYWVSGYQFWL
FTLYDKDEMTDLSNDERKAFAKLLEREVTTRSKQ

Nucleotide

• Length: 345 bp

>T113696 NZ_CP033430:c1804444-1804100 [Burkholderia gladioli]
GTGCAAGCGACCTTCATTGAACTGCCACCGTTTTCTCGTGAACGCGAAAACTACCTGGCAGATGAGGAGTACGCCGTCCC
GCAGAAAGCGCTGCTGGCTAATCCCGAGGCTGGTGATCTGATCAAGGGAGCCGGCAGTCTGCGGAAATTGAGGTTCGCCG
ACAAACGTCGTCAGAAAGGCAAGCGCGGTGGCCTACGAGTGATCTATTACTACTGGGTCAGCGGTTATCAATTCTGGTTG
TTCACGCTGTACGACAAAGATGAAATGACAGACCTCTCCAATGACGAGCGCAAAGCCTTCGCGAAGCTATTGGAAAGAGA
AGTCACGACACGGAGCAAGCAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11456.30 Da
• Isoelectric Point: 10.4005

>AT113696 WP_123036378.1 NZ_CP033430:c1804103-1803789 [Burkholderia gladioli]
MKKRNLFAELTEGLDALAAEHEGKVTLRKITAALPDALEISPAEIKTVREASHASQAVMARSLRINVRTYQNWEQGKAKP
NAQAATLIRLVAKHPETLQMLATL

Nucleotide

• Length: 315 bp

>AT113696 NZ_CP033430:c1804103-1803789 [Burkholderia gladioli]
ATGAAGAAGCGAAATCTGTTTGCTGAATTGACCGAGGGCCTCGATGCCCTTGCCGCTGAGCACGAAGGGAAAGTCACGCT
GCGCAAGATCACAGCCGCTCTGCCCGATGCGCTCGAAATCTCGCCGGCAGAAATCAAGACCGTGCGTGAAGCTTCTCACG
CGTCGCAAGCCGTCATGGCTCGAAGCCTGCGCATCAACGTGCGCACGTATCAGAATTGGGAACAAGGCAAGGCCAAGCCC
AACGCACAGGCGGCTACACTAATCCGACTGGTCGCCAAGCACCCGGAAACACTTCAGATGCTGGCAACTCTCTGA