Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3002558..3003267 Replicon chromosome
Accession NZ_CP033428
Organism Mycobacterium avium subsp. paratuberculosis strain MAPK_CN7/15

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag EC391_RS14335 Protein ID WP_003873387.1
Coordinates 3002863..3003267 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag EC391_RS14330 Protein ID WP_003873388.1
Coordinates 3002558..3002866 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC391_RS14310 2998443..2998745 + 303 WP_003873391.1 co-chaperone GroES -
EC391_RS14315 2998834..3000450 + 1617 WP_003873390.1 chaperonin GroEL -
EC391_RS14320 3000545..3002071 + 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
EC391_RS14330 3002558..3002866 - 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
EC391_RS14335 3002863..3003267 - 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EC391_RS14340 3003507..3004199 + 693 WP_010950286.1 DUF4352 domain-containing protein -
EC391_RS14345 3004254..3005453 + 1200 WP_010950287.1 FAD-dependent oxidoreductase -
EC391_RS14350 3005610..3006374 - 765 WP_003874401.1 superoxide dismutase family protein -
EC391_RS14355 3006417..3006725 - 309 WP_003874400.1 WhiB family transcriptional regulator -
EC391_RS14360 3007123..3007953 + 831 WP_003874399.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T113693 WP_003873387.1 NZ_CP033428:c3003267-3002863 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T113693 NZ_CP033428:c3003267-3002863 [Mycobacterium avium subsp. paratuberculosis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGATGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCAAGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGCAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTACACAAGGCAGCAGCCAAAGCCACCAATCGGAAACCAAGGAAGGGAAAGAAAC
GATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT113693 WP_003873388.1 NZ_CP033428:c3002866-3002558 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT113693 NZ_CP033428:c3002866-3002558 [Mycobacterium avium subsp. paratuberculosis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCAGTACCGTCTCCGCGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTGGGCACGAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References