113686

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1769067..1769776 Replicon chromosome
Accession NZ_CP033427
Organism Mycobacterium avium subsp. paratuberculosis strain MAPK_CN9/15

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag EC390_RS08405 Protein ID WP_003873387.1
Coordinates 1769067..1769471 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag EC390_RS08410 Protein ID WP_003873388.1
Coordinates 1769468..1769776 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC390_RS08380 1764381..1765211 - 831 WP_003874399.1 hypothetical protein -
EC390_RS08385 1765609..1765917 + 309 WP_003874400.1 WhiB family transcriptional regulator -
EC390_RS08390 1765960..1766724 + 765 WP_003874401.1 superoxide dismutase family protein -
EC390_RS08395 1766881..1768080 - 1200 WP_010950287.1 FAD-dependent oxidoreductase -
EC390_RS08400 1768135..1768827 - 693 WP_010950286.1 DUF4352 domain-containing protein -
EC390_RS08405 1769067..1769471 + 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EC390_RS08410 1769468..1769776 + 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
EC390_RS08420 1770263..1771789 - 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
EC390_RS08425 1771884..1773500 - 1617 WP_003873390.1 chaperonin GroEL -
EC390_RS08430 1773589..1773891 - 303 WP_003873391.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T113686 WP_003873387.1 NZ_CP033427:1769067-1769471 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T113686 NZ_CP033427:1769067-1769471 [Mycobacterium avium subsp. paratuberculosis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGATGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCAAGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGCAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTACACAAGGCAGCAGCCAAAGCCACCAATCGGAAACCAAGGAAGGGAAAGAAAC
GATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT113686 WP_003873388.1 NZ_CP033427:1769468-1769776 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT113686 NZ_CP033427:1769468-1769776 [Mycobacterium avium subsp. paratuberculosis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCAGTACCGTCTCCGCGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTGGGCACGAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References