Detailed information of TA system TA113666

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 153890..154143
• Replicon: plasmid pKPC2_115069
• Accession: NZ_CP033404
• Organism: Klebsiella pneumoniae strain WCHKP115069

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: DVJ73_RS02490
• Protein ID: WP_001312851.1
• Coordinates: 153994..154143 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	153890..153949 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVJ73_RS02445	149550..149615	-	66	Protein_187	helix-turn-helix domain-containing protein	-
DVJ73_RS02450	149668..150372	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
DVJ73_RS02455	150436..150597	+	162	Protein_189	DNA helicase	-
DVJ73_RS02460	150617..151363	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
DVJ73_RS02465	151418..151978	+	561	WP_000139328.1	fertility inhibition protein FinO	-
DVJ73_RS02470	152110..152310	+	201	WP_015059022.1	hypothetical protein	-
DVJ73_RS02475	152696..153295	+	600	WP_032083981.1	hypothetical protein	-
DVJ73_RS02480	153459..153689	+	231	WP_001736714.1	hypothetical protein	-
-	153890..153949	-	60	NuclAT_1	-	Antitoxin
-	153890..153949	-	60	NuclAT_1	-	Antitoxin
-	153890..153949	-	60	NuclAT_1	-	Antitoxin
-	153890..153949	-	60	NuclAT_1	-	Antitoxin
DVJ73_RS02490	153994..154143	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
DVJ73_RS02495	154427..154675	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..154986	154986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T113666 WP_001312851.1 NZ_CP033404:153994-154143 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T113666 NZ_CP033404:153994-154143 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT113666 NZ_CP033404:c153949-153890 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin