Detailed information of TA system TA113661

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39794..40064
• Replicon: plasmid pKPC2_115069
• Accession: NZ_CP033404
• Organism: Klebsiella pneumoniae strain WCHKP115069

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DVJ73_RS29880
• Protein ID: WP_001312861.1
• Coordinates: 39906..40064 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39794..39857 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVJ73_RS01650	35505..36032	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
DVJ73_RS01655	36090..36323	+	234	WP_000006003.1	DUF905 family protein	-
DVJ73_RS01660	36384..38407	+	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
DVJ73_RS01665	38476..38910	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DVJ73_RS01670	38907..39626	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39794..39857	-	64	-	-	Antitoxin
DVJ73_RS29880	39906..40064	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DVJ73_RS31155	40302..40679	-	378	Protein_51	hypothetical protein	-
DVJ73_RS01695	40979..41275	+	297	WP_001272251.1	hypothetical protein	-
DVJ73_RS01700	41386..42207	+	822	WP_001234445.1	DUF945 domain-containing protein	-
DVJ73_RS01705	42504..43151	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
DVJ73_RS01710	43428..43811	+	384	WP_000124981.1	relaxosome protein TraM	-
DVJ73_RS01715	44002..44688	+	687	WP_015059009.1	PAS domain-containing protein	-
DVJ73_RS01720	44782..45009	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..154986	154986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T113661 WP_001312861.1 NZ_CP033404:39906-40064 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T113661 NZ_CP033404:39906-40064 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT113661 NZ_CP033404:c39857-39794 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin