Detailed information of TA system TA113630

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54700..54939
• Replicon: plasmid pOXA1_020031
• Accession: NZ_CP033400
• Organism: Escherichia coli strain WCHEC020031

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: EDM51_RS00755
• Protein ID: WP_023144756.1
• Coordinates: 54700..54834 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	54879..54939 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDM51_RS00725	50401..51258	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
EDM51_RS00730	51251..51325	-	75	WP_031943482.1	RepA leader peptide Tap	-
EDM51_RS24230	51322..51456	-	135	Protein_65	protein CopA/IncA	-
EDM51_RS00740	51687..53228	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
EDM51_RS00745	53243..53989	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
EDM51_RS00750	54149..54403	-	255	WP_000083850.1	replication regulatory protein RepA	-
EDM51_RS00755	54700..54834	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	54879..54939	+	61	NuclAT_0	-	Antitoxin
-	54879..54939	+	61	NuclAT_0	-	Antitoxin
-	54879..54939	+	61	NuclAT_0	-	Antitoxin
-	54879..54939	+	61	NuclAT_0	-	Antitoxin
EDM51_RS24075	54906..55192	-	287	Protein_70	DUF2726 domain-containing protein	-
EDM51_RS00770	55705..55917	-	213	WP_013023861.1	hypothetical protein	-
EDM51_RS00775	56048..56608	-	561	WP_000139341.1	fertility inhibition protein FinO	-
EDM51_RS00780	56663..56959	-	297	Protein_73	type-F conjugative transfer system pilin acetylase TraX	-
EDM51_RS00785	56923..58014	-	1092	WP_122950701.1	DUF3560 domain-containing protein	-
EDM51_RS00790	58066..58296	-	231	WP_000218642.1	hypothetical protein	-
EDM51_RS00800	59331..59522	-	192	WP_001027516.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA5 / mph(A) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaOXA-1 / aac(6')-Ib-cr	-	1..91652	91652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T113630 WP_023144756.1 NZ_CP033400:c54834-54700 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T113630 NZ_CP033400:c54834-54700 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT113630 NZ_CP033400:54879-54939 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin