Detailed information of TA system TA113578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3823699..3824240
• Replicon: chromosome
• Accession: NZ_CP033378
• Organism: Escherichia coli strain L73

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q32JN3
• Locus tag: EB670_RS18890
• Protein ID: WP_000615980.1
• Coordinates: 3823962..3824240 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: EB670_RS18885
• Protein ID: WP_000729707.1
• Coordinates: 3823699..3823959 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB670_RS18865	3819365..3820150	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
EB670_RS18870	3820122..3821834	+	1713	Protein_3656	flagellar biosynthesis protein FlhA	-
EB670_RS18875	3822067..3822564	-	498	WP_000006245.1	REP-associated tyrosine transposase RayT	-
EB670_RS18880	3822740..3823513	-	774	WP_000543885.1	C40 family peptidase	-
EB670_RS18885	3823699..3823959	+	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
EB670_RS18890	3823962..3824240	+	279	WP_000615980.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
EB670_RS18895	3824396..3825136	+	741	WP_001225679.1	murein L,D-transpeptidase	-
EB670_RS18900	3825107..3825874	-	768	WP_000333380.1	class II glutamine amidotransferase	-
EB670_RS18905	3826080..3826658	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T113578 WP_000615980.1 NZ_CP033378:3823962-3824240 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T113578 NZ_CP033378:3823962-3824240 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT113578 WP_000729707.1 NZ_CP033378:3823699-3823959 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT113578 NZ_CP033378:3823699-3823959 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGACCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A090NH48