Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1865027..1865529 Replicon chromosome
Accession NZ_CP033378
Organism Escherichia coli strain L73

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EB670_RS09070 Protein ID WP_000767829.1
Coordinates 1865275..1865529 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EB670_RS09065 Protein ID WP_001259255.1
Coordinates 1865027..1865278 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB670_RS09040 1860204..1861271 - 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EB670_RS09045 1861271..1862341 - 1071 WP_001099214.1 histidinol-phosphate transaminase -
EB670_RS09050 1862338..1863642 - 1305 WP_001396164.1 histidinol dehydrogenase -
EB670_RS09055 1863648..1864547 - 900 WP_000566782.1 ATP phosphoribosyltransferase -
EB670_RS09060 1864693..1864743 - 51 WP_001364200.1 his operon leader peptide -
EB670_RS09065 1865027..1865278 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EB670_RS09070 1865275..1865529 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EB670_RS09075 1865612..1866436 + 825 WP_000754754.1 SDR family oxidoreductase -
EB670_RS09080 1866482..1867411 + 930 WP_000803351.1 LysR family transcriptional regulator -
EB670_RS09085 1867626..1867688 + 63 WP_010723108.1 membrane protein YoeI -
EB670_RS09090 1867678..1869036 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EB670_RS09095 1869215..1870273 + 1059 WP_000492305.1 transport protein YeeE -
EB670_RS09100 1870287..1870514 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T113566 WP_000767829.1 NZ_CP033378:1865275-1865529 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T113566 NZ_CP033378:1865275-1865529 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT113566 WP_001259255.1 NZ_CP033378:1865027-1865278 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT113566 NZ_CP033378:1865027-1865278 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References