Detailed information of TA system TA113558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3733553..3734166
• Replicon: chromosome
• Accession: NZ_CP033368
• Organism: Mesorhizobium loti strain SU343

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6M7TIS3
• Locus tag: EFV37_RS18375
• Protein ID: WP_064983789.1
• Coordinates: 3733870..3734166 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EFV37_RS18370
• Protein ID: WP_019859111.1
• Coordinates: 3733553..3733861 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFV37_RS18355	3729442..3730620	-	1179	WP_064983785.1	lytic murein transglycosylase	-
EFV37_RS18360	3730977..3731777	-	801	WP_032930832.1	glycoside hydrolase family 25 protein	-
EFV37_RS18365	3732059..3733438	+	1380	WP_064983787.1	serine hydrolase	-
EFV37_RS18370	3733553..3733861	-	309	WP_019859111.1	HigA family addiction module antidote protein	Antitoxin
EFV37_RS18375	3733870..3734166	-	297	WP_064983789.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EFV37_RS18380	3734285..3734968	-	684	WP_064983791.1	class I SAM-dependent methyltransferase	-
EFV37_RS18385	3734965..3736263	-	1299	WP_064983792.1	DUF3419 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11652.09 Da
• Isoelectric Point: 6.4829

>T113558 WP_064983789.1 NZ_CP033368:c3734166-3733870 [Mesorhizobium loti]
MIVGFRDGWLRAFFVDDTHSRNIPSDLESRLFRKLQMIDDAMVDQDLRVPPSNHFEKLRGNLEGFHSIRVNQQWRLIFRW
DGGRGEASDIYLDDHSYK

Nucleotide

• Length: 297 bp

>T113558 NZ_CP033368:c3734166-3733870 [Mesorhizobium loti]
ATGATCGTCGGTTTTAGAGACGGATGGCTGCGGGCCTTCTTTGTAGACGACACCCACTCCCGCAACATTCCGTCCGATCT
CGAAAGCCGCTTGTTCCGCAAGCTCCAGATGATTGACGACGCCATGGTCGATCAGGATTTGCGCGTGCCGCCCAGCAACC
ATTTCGAGAAGTTGCGCGGCAATCTCGAGGGCTTCCATTCGATCCGCGTCAACCAGCAGTGGCGACTGATCTTCCGCTGG
GACGGCGGTCGCGGTGAAGCGTCAGACATTTATCTCGACGACCACAGTTATAAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11332.09 Da
• Isoelectric Point: 9.4051

>AT113558 WP_019859111.1 NZ_CP033368:c3733861-3733553 [Mesorhizobium loti]
MLMTARKPATVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLPTAA

Nucleotide

• Length: 309 bp

>AT113558 NZ_CP033368:c3733861-3733553 [Mesorhizobium loti]
ATGTTGATGACCGCACGCAAGCCAGCAACGGTTGGCGAGATTCTCACCGAGGAGTTCATGCAGCCGCTCGGCTTGACGCA
GGCGGCATTGGCCGAGGCGATGGGCGTTCAGCGCAAGCATGTGAATGAATTGTGCAACGACCGTCGCAACGTGACGGCGG
CAACCGCGCTCATTCTGGCCCGGGTGTTTGGCAACAGCCCGGACTTCTGGCTGAATGTCCAACGCCGCAGCGATCTTTGG
GCGGTTATGAATTCGCCCGACGAGCGGGCACGTGTTGACCGCGCCAAGCCTTTACCGACAGCCGCATAG

Structures

Toxin

• AlphaFold DB: A0A6M7TIS3