Detailed information of TA system TA113556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3614918..3615533
• Replicon: chromosome
• Accession: NZ_CP033367
• Organism: Mesorhizobium loti R88b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6M7WVT3
• Locus tag: EB235_RS17680
• Protein ID: WP_027029733.1
• Coordinates: 3615237..3615533 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EB235_RS17675
• Protein ID: WP_027029734.1
• Coordinates: 3614918..3615226 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB235_RS17660	3611061..3612239	-	1179	WP_027029737.1	lytic murein transglycosylase	-
EB235_RS17665	3612482..3613279	-	798	WP_027029736.1	glycoside hydrolase family 25 protein	-
EB235_RS17670	3613523..3614902	+	1380	WP_027029735.1	serine hydrolase	-
EB235_RS17675	3614918..3615226	-	309	WP_027029734.1	HigA family addiction module antidote protein	Antitoxin
EB235_RS17680	3615237..3615533	-	297	WP_027029733.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EB235_RS17685	3615645..3616304	-	660	WP_027029732.1	class I SAM-dependent methyltransferase	-
EB235_RS17690	3616301..3617599	-	1299	WP_027029731.1	DUF3419 family protein	-
EB235_RS17695	3617631..3618176	-	546	WP_208603620.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11656.06 Da
• Isoelectric Point: 7.3180

>T113556 WP_027029733.1 NZ_CP033367:c3615533-3615237 [Mesorhizobium loti R88b]
MIVDFRDGWLRAYFVDNTRSRNIPSDLESRLFRKLQMIDDAAIDQDLRAPPSNHFEKLRGNLEGFHSIRVNKQWRLVFRW
DGGRGEASDIYLDDHSYK

Nucleotide

• Length: 297 bp

>T113556 NZ_CP033367:c3615533-3615237 [Mesorhizobium loti R88b]
ATGATCGTCGACTTTAGAGACGGATGGCTGCGGGCCTACTTTGTAGACAATACCCGCTCCCGCAACATTCCGTCCGATCT
CGAAAGCCGCTTGTTCCGAAAGCTCCAGATGATTGACGATGCGGCGATTGACCAAGATTTGCGTGCTCCGCCCAGCAATC
ATTTCGAGAAGTTGCGCGGCAATCTCGAAGGCTTCCATTCTATCCGCGTCAACAAACAATGGCGTCTGGTCTTCCGGTGG
GACGGCGGTCGCGGTGAGGCGTCGGACATCTATCTCGACGACCACAGTTACAAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.08 Da
• Isoelectric Point: 10.4486

>AT113556 WP_027029734.1 NZ_CP033367:c3615226-3614918 [Mesorhizobium loti R88b]
MLMTTRKPATVGEILTEEFMQPLGLTQAALAGAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
TVMNSPDERARVDRAKPLPTAA

Nucleotide

• Length: 309 bp

>AT113556 NZ_CP033367:c3615226-3614918 [Mesorhizobium loti R88b]
ATGTTGATGACCACACGCAAACCAGCAACGGTTGGCGAAATTCTCACCGAGGAATTCATGCAGCCGCTTGGCTTGACGCA
GGCGGCTTTGGCCGGGGCGATGGGCGTTCAGCGCAAGCATGTGAATGAATTGTGCAACGACCGCCGCAACGTGACAGCGG
CAACCGCGCTCATTCTGGCACGGGTGTTTGGCAACAGCCCCGACTTCTGGCTGAACGTCCAACGCCGCAGCGATCTTTGG
ACGGTCATGAATTCGCCCGACGAACGGGCGCGTGTCGACCGCGCCAAGCCTTTACCGACAGCCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A6M7WVT3