Detailed information of TA system TA113550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3072770..3073383
• Replicon: chromosome
• Accession: NZ_CP033366
• Organism: Mesorhizobium japonicum R7A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Q1US98
• Locus tag: EB234_RS15130
• Protein ID: WP_032930829.1
• Coordinates: 3073087..3073383 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EB234_RS15125
• Protein ID: WP_032930830.1
• Coordinates: 3072770..3073078 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB234_RS15110	3068633..3069811	-	1179	WP_032930833.1	lytic murein transglycosylase	-
EB234_RS15115	3070226..3071026	-	801	WP_032930832.1	glycoside hydrolase family 25 protein	-
EB234_RS15120	3071315..3072694	+	1380	WP_032930831.1	serine hydrolase	-
EB234_RS15125	3072770..3073078	-	309	WP_032930830.1	HigA family addiction module antidote protein	Antitoxin
EB234_RS15130	3073087..3073383	-	297	WP_032930829.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EB234_RS31805	3073449..3073643	-	195	WP_080680274.1	hypothetical protein	-
EB234_RS15140	3073696..3074181	-	486	WP_032930828.1	hypothetical protein	-
EB234_RS15145	3074357..3075040	-	684	WP_032930827.1	class I SAM-dependent methyltransferase	-
EB234_RS15150	3075037..3076335	-	1299	WP_032930826.1	DUF3419 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11583.01 Da
• Isoelectric Point: 7.3192

>T113550 WP_032930829.1 NZ_CP033366:c3073383-3073087 [Mesorhizobium japonicum R7A]
MIVGFRDGWLRAFFVDDTRSRNIPSDLESRLFRKLQMIDDATVDQDLRVPPSNHFEKLRGNLEGFHSIRVNQQWRLIFRW
GGGRGEASDIYLDDHSYK

Nucleotide

• Length: 297 bp

>T113550 NZ_CP033366:c3073383-3073087 [Mesorhizobium japonicum R7A]
ATGATCGTCGGTTTTAGAGACGGATGGCTGCGGGCTTTCTTTGTAGACGACACCCGCTCCCGCAACATTCCGTCCGATCT
CGAAAGCCGCTTGTTCCGCAAGCTCCAGATGATTGACGACGCCACGGTCGATCAGGATTTGCGCGTACCGCCCAGCAACC
ATTTCGAGAAGTTGCGCGGCAATCTCGAAGGCTTCCATTCGATCCGCGTCAACCAGCAGTGGCGATTGATCTTCCGCTGG
GGCGGCGGTCGCGGTGAAGCGTCGGACATCTATCTCGACGACCACAGTTATAAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11306.05 Da
• Isoelectric Point: 9.4051

>AT113550 WP_032930830.1 NZ_CP033366:c3073078-3072770 [Mesorhizobium japonicum R7A]
MLMTARKPATVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLATAA

Nucleotide

• Length: 309 bp

>AT113550 NZ_CP033366:c3073078-3072770 [Mesorhizobium japonicum R7A]
ATGTTGATGACCGCACGCAAGCCAGCAACGGTTGGCGAGATTCTCACCGAAGAGTTCATGCAGCCGCTCGGCTTGACGCA
GGCGGCTTTGGCCGAGGCGATGGGCGTTCAGCGCAAGCATGTGAACGAATTGTGCAACGACCGCCGCAACGTGACGGCGG
CAACCGCGCTCATTCTGGCCCGGGTGTTTGGCAACAGCCCGGACTTCTGGCTGAACGTCCAACGCCGCAGCGATCTTTGG
GCGGTTATGAATTCGCCCGACGAGCGGGCACGTGTTGACCGCGCCAAGCCTTTGGCGACAGCCGCATAG

Structures

Toxin

• AlphaFold DB: A0A4Q1US98