Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2414539..2415134 Replicon chromosome
Accession NZ_CP033361
Organism Mesorhizobium erdmanii strain NZP2014

Toxin (Protein)


Gene name graT Uniprot ID A0A6M7UG11
Locus tag EB233_RS11675 Protein ID WP_064989650.1
Coordinates 2414853..2415134 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A6M7UFT9
Locus tag EB233_RS11670 Protein ID WP_064989651.1
Coordinates 2414539..2414838 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB233_RS11635 2409721..2410125 + 405 WP_064989738.1 peptidase -
EB233_RS11640 2410142..2410759 - 618 WP_064989654.1 flavodoxin family protein -
EB233_RS11645 2411005..2412639 + 1635 WP_064989653.1 tetratricopeptide repeat-containing sulfotransferase family protein -
EB233_RS11650 2412750..2413103 - 354 WP_064989652.1 DUF1428 domain-containing protein -
EB233_RS11655 2413214..2413636 - 423 WP_064989737.1 glyoxalase/bleomycin resistance/extradiol dioxygenase family protein -
EB233_RS11660 2413791..2413973 - 183 WP_032917983.1 hypothetical protein -
EB233_RS11665 2414067..2414453 - 387 WP_027055070.1 nuclear transport factor 2 family protein -
EB233_RS11670 2414539..2414838 - 300 WP_064989651.1 HigA family addiction module antidote protein Antitoxin
EB233_RS11675 2414853..2415134 - 282 WP_064989650.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EB233_RS11680 2415251..2416777 - 1527 WP_064989649.1 ATP-binding cassette domain-containing protein -
EB233_RS11685 2417019..2417924 - 906 WP_064989648.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-68)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10575.99 Da        Isoelectric Point: 9.8330

>T113537 WP_064989650.1 NZ_CP033361:c2415134-2414853 [Mesorhizobium erdmanii]
MIRSFKDKTTEAVSNGKAPKGFPSDLLRSAQRKLFMIDNATELSDLKTPPGNRLEPLKGSRAGQHSIRINDQFRICFRWL
DGQAENVEITDYH

Download         Length: 282 bp

>T113537 NZ_CP033361:c2415134-2414853 [Mesorhizobium erdmanii]
ATGATACGCTCGTTCAAGGACAAGACCACCGAGGCCGTTTCGAACGGCAAGGCTCCAAAGGGGTTCCCATCGGACCTTCT
CCGATCAGCCCAACGGAAACTGTTCATGATCGACAACGCGACCGAGTTGTCGGACCTTAAGACGCCTCCCGGCAACAGGC
TTGAGCCCCTGAAAGGCAGCCGTGCCGGTCAGCATTCGATCCGCATCAACGATCAGTTTCGCATCTGCTTTCGCTGGTTG
GACGGACAAGCCGAGAATGTCGAAATAACCGACTACCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11122.89 Da        Isoelectric Point: 5.7134

>AT113537 WP_064989651.1 NZ_CP033361:c2414838-2414539 [Mesorhizobium erdmanii]
MLDINPPIHPGEILREEYLVPLGMKPYSLAKRLHVPRTRIERLASEATPVTPDTALRLAKFFGTTPQFWMNMQASYDLAV
EGEAKKADLDAIERMEAAA

Download         Length: 300 bp

>AT113537 NZ_CP033361:c2414838-2414539 [Mesorhizobium erdmanii]
ATGCTTGATATCAACCCGCCTATCCATCCCGGAGAAATCCTTCGAGAGGAATACCTCGTCCCGCTTGGCATGAAGCCCTA
TTCACTGGCCAAAAGACTGCATGTGCCACGCACACGCATCGAGCGCTTGGCCAGCGAGGCAACACCGGTGACGCCGGACA
CCGCGCTACGCCTTGCCAAGTTTTTCGGAACCACGCCGCAGTTCTGGATGAACATGCAGGCGAGCTACGACCTTGCCGTC
GAGGGCGAGGCCAAGAAGGCCGACCTTGATGCGATCGAACGCATGGAGGCCGCGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M7UG11

Antitoxin

Source ID Structure
AlphaFold DB A0A6M7UFT9

References