Detailed information of TA system TA113512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 92051..92640
• Replicon: chromosome
• Accession: NZ_CP033326
• Organism: Xanthomonas cucurbitae strain ATCC 23378

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: EBN15_RS00395
• Protein ID: WP_104603499.1
• Coordinates: 92051..92332 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBN15_RS00400
• Protein ID: WP_104603498.1
• Coordinates: 92350..92640 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBN15_RS00390	89767..91953	+	2187	WP_159406426.1	exodeoxyribonuclease V subunit alpha	-
EBN15_RS00395	92051..92332	+	282	WP_104603499.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBN15_RS00400	92350..92640	+	291	WP_104603498.1	HigA family addiction module antidote protein	Antitoxin
EBN15_RS00410	93608..94141	+	534	WP_208800435.1	DUF3800 domain-containing protein	-
EBN15_RS00415	94346..94678	-	333	WP_104603496.1	hypothetical protein	-
EBN15_RS00420	95278..95607	+	330	WP_159406427.1	DUF1778 domain-containing protein	-
EBN15_RS00425	95555..96100	+	546	WP_159406428.1	GNAT family N-acetyltransferase	-
EBN15_RS00430	96167..96370	+	204	Protein_82	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	80233..96100	15867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11012.71 Da
• Isoelectric Point: 10.2909

>T113512 WP_104603499.1 NZ_CP033326:92051-92332 [Xanthomonas cucurbitae]
MIRSFIDKEAERIWLGERSRRLPADIQPVARRKLRMLNAAAHLDDLRIPPANRLEALKGARRGQYSIRINDQWRICFRWM
EGDAAEVEIVDYH

Nucleotide

• Length: 282 bp

>T113512 NZ_CP033326:92051-92332 [Xanthomonas cucurbitae]
ATGATTAGAAGCTTTATCGACAAGGAAGCCGAAAGGATCTGGTTGGGTGAGCGCTCCCGCCGGCTGCCGGCCGACATCCA
GCCGGTGGCGCGCCGCAAGTTGCGCATGCTCAACGCTGCCGCGCACCTTGACGATTTACGCATTCCGCCCGCCAACAGGC
TGGAGGCATTGAAAGGCGCACGGCGCGGCCAGTACAGCATCCGGATCAACGACCAGTGGCGCATCTGCTTTCGATGGATG
GAAGGCGATGCAGCCGAGGTCGAGATAGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10464.13 Da
• Isoelectric Point: 7.2043

>AT113512 WP_104603498.1 NZ_CP033326:92350-92640 [Xanthomonas cucurbitae]
MTVLPNIHPGEILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTTERFWLGLQADYELEQA
HRALGDLPSRIKRLAA

Nucleotide

• Length: 291 bp

>AT113512 NZ_CP033326:92350-92640 [Xanthomonas cucurbitae]
ATGACAGTCCTACCCAACATTCATCCTGGCGAAATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ATTGGCGCGGGCCACCGGCGTGCCGCCGCGCCGCATCAATGAAATCGTACTGGGCAAGCGTGGCATCACCGCCGACACCG
CCGTGCGCCTGGCTGCAGCGCTGGGAACCACCGAGCGCTTCTGGCTGGGCCTGCAAGCCGACTACGAACTGGAACAAGCG
CATCGCGCGCTGGGCGATCTGCCGTCACGGATCAAGCGGCTGGCGGCTTGA