Detailed information of TA system TA113500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3817621..3818315
• Replicon: chromosome
• Accession: NZ_CP033250
• Organism: Escherichia coli strain ECCHD184

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: D3W51_RS18665
• Protein ID: WP_001263489.1
• Coordinates: 3817621..3818019 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: D3W51_RS18670
• Protein ID: WP_000554758.1
• Coordinates: 3818022..3818315 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3813209..3813289	-	81	NuclAT_12	-	-
-	3813209..3813289	-	81	NuclAT_12	-	-
-	3813209..3813289	-	81	NuclAT_12	-	-
-	3813209..3813289	-	81	NuclAT_12	-	-
D3W51_RS18640	3813885..3814343	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D3W51_RS18645	3814604..3816061	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
D3W51_RS18650	3816118..3816639	-	522	Protein_3621	peptide chain release factor H	-
D3W51_RS18655	3816635..3816841	-	207	Protein_3622	RtcB family protein	-
D3W51_RS18660	3817159..3817611	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
D3W51_RS18665	3817621..3818019	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D3W51_RS18670	3818022..3818315	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D3W51_RS18675	3818367..3819422	-	1056	WP_001226164.1	DNA polymerase IV	-
D3W51_RS18680	3819493..3820278	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
D3W51_RS18685	3820250..3821962	+	1713	Protein_3628	flagellar biosynthesis protein FlhA	-
D3W51_RS18690	3822186..3822683	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T113500 WP_001263489.1 NZ_CP033250:c3818019-3817621 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T113500 NZ_CP033250:c3818019-3817621 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT113500 WP_000554758.1 NZ_CP033250:c3818315-3818022 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT113500 NZ_CP033250:c3818315-3818022 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3