Detailed information of TA system TA113484

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2165116..2165487
• Replicon: chromosome
• Accession: NZ_CP033250
• Organism: Escherichia coli strain ECCHD184

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: D3W51_RS10405
• Protein ID: WP_001317028.1
• Coordinates: 2165293..2165487 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2165116..2165294 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W51_RS10380	2161071..2162444	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
D3W51_RS10385	2162573..2163508	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
D3W51_RS10390	2163560..2164795	-	1236	WP_000040852.1	site-specific integrase	-
D3W51_RS10395	2164797..2165012	-	216	WP_000079604.1	excisionase XisR	-
D3W51_RS10400	2165091..2165300	-	210	WP_053904466.1	double-strand break reduction protein RcbA	-
-	2165116..2165294	+	179	NuclAT_0	-	Antitoxin
-	2165116..2165294	+	179	NuclAT_0	-	Antitoxin
-	2165116..2165294	+	179	NuclAT_0	-	Antitoxin
-	2165116..2165294	+	179	NuclAT_0	-	Antitoxin
D3W51_RS10405	2165293..2165487	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
D3W51_RS10410	2165544..2166353	-	810	WP_000166319.1	recombination protein RecT	-
D3W51_RS10415	2166346..2168946	-	2601	WP_001532611.1	exodeoxyribonuclease VIII	-
D3W51_RS10420	2169048..2169323	-	276	WP_122224624.1	hypothetical protein	-
D3W51_RS10425	2169398..2169568	-	171	WP_001352098.1	conserved protein, Rac prophage	-
D3W51_RS10430	2169568..2169789	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T113484 WP_001317028.1 NZ_CP033250:c2165487-2165293 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T113484 NZ_CP033250:c2165487-2165293 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT113484 NZ_CP033250:2165116-2165294 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGACGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin