Detailed information of TA system TA113424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3645013..3645530
• Replicon: chromosome
• Accession: NZ_CP033230
• Organism: Sphingobium yanoikuyae strain SJTF8

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EBF16_RS20895
• Protein ID: WP_122129922.1
• Coordinates: 3645264..3645530 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A085K6H7
• Locus tag: EBF16_RS20890
• Protein ID: WP_017500001.1
• Coordinates: 3645013..3645267 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBF16_RS20865	3640293..3642428	+	2136	WP_037508150.1	PKD domain-containing protein	-
EBF16_RS20870	3642469..3642999	-	531	WP_037508148.1	hypothetical protein	-
EBF16_RS20875	3643146..3643589	+	444	WP_037508146.1	hypothetical protein	-
EBF16_RS20880	3643731..3644390	+	660	WP_037508145.1	hypothetical protein	-
EBF16_RS20885	3644600..3644896	-	297	WP_037508143.1	hypothetical protein	-
EBF16_RS20890	3645013..3645267	+	255	WP_017500001.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
EBF16_RS20895	3645264..3645530	+	267	WP_122129922.1	Txe/YoeB family addiction module toxin	Toxin
EBF16_RS28870	3645532..3645900	-	369	WP_155282050.1	hypothetical protein	-
EBF16_RS20900	3645890..3646396	-	507	WP_080726988.1	ImmA/IrrE family metallo-endopeptidase	-
EBF16_RS20905	3646519..3648414	-	1896	WP_037508141.1	phosphomethylpyrimidine synthase ThiC	-
EBF16_RS20910	3648753..3648971	+	219	WP_052076578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3364272..3743590	379318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10087.48 Da
• Isoelectric Point: 9.6015

>T113424 WP_122129922.1 NZ_CP033230:3645264-3645530 [Sphingobium yanoikuyae]
VKIVFGSQAWRDYVALAADDPKVLARINALIEECRRDPFRGTGKPEPLRQNLAGWWSRRITGEHRLVYRVSGSGDTQALE
VLSCRYHY

Nucleotide

• Length: 267 bp

>T113424 NZ_CP033230:3645264-3645530 [Sphingobium yanoikuyae]
GTGAAGATCGTCTTCGGATCGCAGGCATGGCGCGACTATGTCGCCTTGGCCGCGGACGATCCCAAGGTGCTGGCGCGGAT
CAACGCGCTGATCGAGGAATGCCGCCGCGATCCGTTTCGCGGCACGGGCAAGCCGGAGCCATTGCGGCAGAACCTGGCCG
GCTGGTGGTCAAGGCGCATCACCGGCGAGCATCGGCTGGTCTATCGGGTCAGCGGATCAGGCGACACGCAGGCGCTGGAA
GTGCTGAGTTGCCGGTATCATTATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9023.31 Da
• Isoelectric Point: 4.5448

>AT113424 WP_017500001.1 NZ_CP033230:3645013-3645267 [Sphingobium yanoikuyae]
MQTVSVSETRANLKAVLDRVVADKAPIQIVRPKGEGVVMISQSEWEGMQETLHLLASPANAQHLLEGIGQLDAGLGEEHE
LIAP

Nucleotide

• Length: 255 bp

>AT113424 NZ_CP033230:3645013-3645267 [Sphingobium yanoikuyae]
ATGCAGACCGTCAGCGTTTCCGAAACCCGCGCCAATCTCAAGGCCGTGCTCGACCGTGTTGTCGCCGACAAGGCGCCGAT
CCAGATCGTGCGGCCCAAGGGCGAAGGGGTGGTGATGATTTCGCAAAGCGAATGGGAGGGCATGCAGGAGACGCTGCACC
TGTTGGCATCGCCCGCCAATGCCCAGCATCTGCTGGAGGGCATCGGGCAACTGGATGCCGGGCTCGGCGAGGAGCATGAG
CTGATCGCGCCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A085K6H7