Detailed information of TA system TA113392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3847491..3848080
• Replicon: chromosome
• Accession: NZ_CP033201
• Organism: Xanthomonas oryzae pv. oryzae strain BXO1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: BXO1_RS19120
• Protein ID: WP_012443754.1
• Coordinates: 3847491..3847772 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BXO1_RS19125
• Protein ID: WP_011260746.1
• Coordinates: 3847790..3848080 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO1_RS19115 (BXO1_019030)	3845137..3847396	+	2260	Protein_3431	exodeoxyribonuclease V subunit alpha	-
BXO1_RS19120 (BXO1_019035)	3847491..3847772	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BXO1_RS19125 (BXO1_019040)	3847790..3848080	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
BXO1_RS19135 (BXO1_019050)	3849009..3850403	+	1395	WP_075240796.1	type III secretion system effector XopQ	-
BXO1_RS25920	3850506..3850693	-	188	Protein_3435	hypothetical protein	-
BXO1_RS19145 (BXO1_019060)	3850853..3851185	-	333	WP_011260743.1	hypothetical protein	-
BXO1_RS19155 (BXO1_019070)	3851440..3851766	+	327	WP_237384726.1	hypothetical protein	-
BXO1_RS19160 (BXO1_019075)	3851894..3852316	+	423	WP_033013301.1	PAAR domain-containing protein	-
BXO1_RS19165 (BXO1_019080)	3852319..3852795	+	477	WP_011260741.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T113392 WP_012443754.1 NZ_CP033201:3847491-3847772 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T113392 NZ_CP033201:3847491-3847772 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT113392 WP_011260746.1 NZ_CP033201:3847790-3848080 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT113392 NZ_CP033201:3847790-3848080 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGGGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5