Detailed information of TA system TA113385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4790487..4791076
• Replicon: chromosome
• Accession: NZ_CP033197
• Organism: Xanthomonas oryzae pv. oryzae strain KXO85

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A8E2YMU3
• Locus tag: EBA20_RS23920
• Protein ID: WP_011260747.1
• Coordinates: 4790795..4791076 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBA20_RS23915
• Protein ID: WP_011260746.1
• Coordinates: 4790487..4790777 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA20_RS23875	4785762..4786238	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
EBA20_RS23880	4786241..4786675	-	435	WP_011260742.1	PAAR domain-containing protein	-
EBA20_RS23885	4786813..4787037	-	225	WP_044751358.1	hypothetical protein	-
EBA20_RS23895	4787373..4787705	+	333	WP_011260743.1	hypothetical protein	-
EBA20_RS23905	4788155..4789549	-	1395	WP_011260745.1	nucleoside hydrolase	-
EBA20_RS23915	4790487..4790777	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
EBA20_RS23920	4790795..4791076	-	282	WP_011260747.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBA20_RS23925	4791171..4793429	-	2259	WP_041182333.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11070.83 Da
• Isoelectric Point: 10.2139

>T113385 WP_011260747.1 NZ_CP033197:c4791076-4790795 [Xanthomonas oryzae pv. oryzae]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQQRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T113385 NZ_CP033197:c4791076-4790795 [Xanthomonas oryzae pv. oryzae]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT113385 WP_011260746.1 NZ_CP033197:c4790777-4790487 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT113385 NZ_CP033197:c4790777-4790487 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA