Detailed information of TA system TA113381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4765764..4766353
• Replicon: chromosome
• Accession: NZ_CP033196
• Organism: Xanthomonas oryzae pv. oryzae strain AUST2013

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: EBA17_RS23790
• Protein ID: WP_012443754.1
• Coordinates: 4766072..4766353 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBA17_RS23785
• Protein ID: WP_133265415.1
• Coordinates: 4765764..4766054 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA17_RS23745	4761048..4761524	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
EBA17_RS23750	4761527..4761961	-	435	WP_011260742.1	PAAR domain-containing protein	-
EBA17_RS23755	4762099..4762323	-	225	WP_044751358.1	hypothetical protein	-
EBA17_RS23765	4762659..4762991	+	333	WP_011260743.1	hypothetical protein	-
EBA17_RS23775	4763441..4764835	-	1395	WP_011260745.1	nucleoside hydrolase	-
EBA17_RS23785	4765764..4766054	-	291	WP_133265415.1	HigA family addiction module antidote protein	Antitoxin
EBA17_RS23790	4766072..4766353	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBA17_RS23795	4766448..4768700	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T113381 WP_012443754.1 NZ_CP033196:c4766353-4766072 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T113381 NZ_CP033196:c4766353-4766072 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10447.14 Da
• Isoelectric Point: 8.7293

>AT113381 WP_133265415.1 NZ_CP033196:c4766054-4765764 [Xanthomonas oryzae pv. oryzae]
MKVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT113381 NZ_CP033196:c4766054-4765764 [Xanthomonas oryzae pv. oryzae]
ATGAAGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5